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Efficient Labeling of Porcine Hematopoietic Cells by Fluorescence-Conjugated Nanoparticles  

Lee, Hyun-Joo (Dept. of Biomedical Science, College of Life Science, CHA University)
Park, Eun-Ji (Dept. of Biomedical Science, College of Life Science, CHA University)
Lee, Yong-Soo (Dept. of Biomedical Science, College of Life Science, CHA University)
Park, Sung-Won (Dept. of Biomedical Science, College of Life Science, CHA University)
Kim, Jae-Hwan (Dept. of Biomedical Science, College of Life Science, CHA University)
Kim, Dong-Ku (Dept. of Biomedical Science, College of Life Science, CHA University)
Publication Information
Reproductive and Developmental Biology / v.34, no.3, 2010 , pp. 175-180 More about this Journal
Abstract
Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.
Keywords
Silica-coated nanoparticles; Porcine hematopoietic cells; RITC; Biocompatibility; Confocal microscopy;
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