식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제40권3호
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  • Pages.157-162
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  • 2010
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
권호 표지

섬기린초에서 엽록체 DNA 염기서열의 종내 변이와 지리적 분포 양상 연구

Intraspecific sequence variation of trnL/F intergenic region (cpDNA) in Sedum takesimense Nakai (Crassulaceae) and aspects of geographic distribution

  • 이웅 (경북대학교 자연과학대학 생물학과 및 울릉도.독도 연구소) ;
  • 박재홍 (경북대학교 자연과학대학 생물학과 및 울릉도.독도 연구소)
  • Lee, Woong (Department of Biology, College of Natural Science, Kyungpook National University, Research Institute for Dokdo and Ulleungdo Island, Kyungpook National University) ;
  • Pak, Jae-Hong (Department of Biology, College of Natural Science, Kyungpook National University, Research Institute for Dokdo and Ulleungdo Island, Kyungpook National University)
  • 투고 : 2010.07.27
  • 심사 : 2010.09.08
  • 발행 : 2010.09.30
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초록

우리나라의 울릉도와 독도에 분포하는 한국특산종인 섬기린초는 해안암석지대를 터전으로 넓게 분포하여 울릉도 생태계의 중요한 부분을 차지하고 있다. 본 연구는 섬기린초에 대한 엽록체 DNA의 trnL/F intergenic spacer 염기서열을 총 32개체에 대하여 조사하였다. 그 결과, 정렬된 염기서열 중 하나의 6-bp indel (-ATTCAC-)에 의하여 두 개의 type (TYPE01: 297bp과 TYPE02: 291bp)을 확인하였다. 확인된 두 개의 엽록체 DNA type은 울릉도와 독도에서 뚜렷한 지리적 분포양상을 보여주었다. TYPE01은 울릉도(15개체)에서만 관찰되었고 TYPE02는 울릉도(12개체)와 독도(5개체)에서 확인되었다. 섬기린초는 하나의 6-bp indel에 의하여 서로 다른 두 개의 엽록체 DNA haplotype이 확인되고 뚜렷한 지리적 분포양상을 보여주기 때문에, 울릉도와 독도에 자생하는 개체군 내에 진화적으로 서로 다른 두 개의 계통이 있음을 추정할 수 있고 울릉도와 독도 간 원거리 분산 기작의 가능성을 지지하였다.

Sequences of the trnL/F intergenic spacer of chloroplast DNA were used to investigate the intraspecific evoution and phylogeography of Sedum takesimense (Crassulaceae). The trnL/F intergeneric spacer sequences from 32 individuals of S. takesimense were either 291 bp (17 samples "without indel" in the following) or 297 bp (15samples "with indel 1") in length due to an indel of 6 bp. Two main cpDNA haplotypes were detected within S. takesimense. The haplotype with indel was found on Ulleung Island and without indel on Ulleung Island and Dok Island. This confirmed the existence of two cpDNA lineages with different geographical distributions. The cpDNA sequence analysis also suggested a putative long-distance dispersal event between Ulleung Island and Dok Island.

키워드

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