시화호 인공습지의 수질정화 및 암모니아 산화균의 분포 연구

The Efficacy of Water Purification and Distribution of Ammonia Oxidizing Bacteria in Shihwa Constructed Wetland

  • 김세윤 (서울대학교 보건대학원 환경보건학과) ;
  • 김미순 (서울대학교 보건대학원 환경보건학과) ;
  • 이성희 (서울대학교 보건대학원 환경보건학과) ;
  • 임미영 (서울대학교 보건대학원 환경보건학과) ;
  • 이영민 (서울대학교 보건대학원 환경보건학과) ;
  • 김지열 (서울대학교 보건대학원 환경보건학과) ;
  • 고광표 (서울대학교 보건대학원 환경보건학과)
  • Kim, Seiyoon (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Kim, Misoon (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Lee, Sunghee (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Lim, Miyoung (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Lee, Youngmin (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Kim, Zhiyeol (Department of Environmental Health, School of Public Health, Seoul National University) ;
  • Ko, GwangPyo (Department of Environmental Health, School of Public Health, Seoul National University)
  • 투고 : 2009.04.24
  • 심사 : 2009.12.14
  • 발행 : 2010.01.30

초록

Water quality and the distribution of ammonia oxidizing bacteria were characterized in constructed wetland of Shihwa lake. Both physico-chemical parameters and fecal indicator microorganisms including total coliforms, E.coli, Enterococcus spp. were measured. In addition, denaturant gradient gel electrophoresis (DGGE) was carried out after PCR amplification of amoA gene from input, output, and wetland sites of the Banwol, Donghwa, and Samhwa stream in Shihwa lake area. Physico-chemical parameters were in proper range for typical nitrifying bacteria to grow and perform their biological activities. Average concentrations of fecal indicator microorganisms of wetland samples were lower than those of input sites. These results suggested that microbial water quality improved by the process of constructed wetland. According to phylogenetic information obtained from DGGE from study sites, distribution of nitrifying bacteria from each of input, output, and wetland were generally distinctive one another. In addition, distribution of nitrifying bacteria between Banwol and Donghwa streams showed higher similarity (52.6%) than this of Samhwa stream (15.2%). These results indicated that characteristics of ammonia oxidizing bacteria in Samhwa were unique in comparison with those of Banwol and Donghwa stream.

키워드

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