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The Efficacy of Water Purification and Distribution of Ammonia Oxidizing Bacteria in Shihwa Constructed Wetland  

Kim, Seiyoon (Department of Environmental Health, School of Public Health, Seoul National University)
Kim, Misoon (Department of Environmental Health, School of Public Health, Seoul National University)
Lee, Sunghee (Department of Environmental Health, School of Public Health, Seoul National University)
Lim, Miyoung (Department of Environmental Health, School of Public Health, Seoul National University)
Lee, Youngmin (Department of Environmental Health, School of Public Health, Seoul National University)
Kim, Zhiyeol (Department of Environmental Health, School of Public Health, Seoul National University)
Ko, GwangPyo (Department of Environmental Health, School of Public Health, Seoul National University)
Publication Information
Journal of Korean Society on Water Environment / v.26, no.1, 2010 , pp. 10-18 More about this Journal
Abstract
Water quality and the distribution of ammonia oxidizing bacteria were characterized in constructed wetland of Shihwa lake. Both physico-chemical parameters and fecal indicator microorganisms including total coliforms, E.coli, Enterococcus spp. were measured. In addition, denaturant gradient gel electrophoresis (DGGE) was carried out after PCR amplification of amoA gene from input, output, and wetland sites of the Banwol, Donghwa, and Samhwa stream in Shihwa lake area. Physico-chemical parameters were in proper range for typical nitrifying bacteria to grow and perform their biological activities. Average concentrations of fecal indicator microorganisms of wetland samples were lower than those of input sites. These results suggested that microbial water quality improved by the process of constructed wetland. According to phylogenetic information obtained from DGGE from study sites, distribution of nitrifying bacteria from each of input, output, and wetland were generally distinctive one another. In addition, distribution of nitrifying bacteria between Banwol and Donghwa streams showed higher similarity (52.6%) than this of Samhwa stream (15.2%). These results indicated that characteristics of ammonia oxidizing bacteria in Samhwa were unique in comparison with those of Banwol and Donghwa stream.
Keywords
amoA; Constructed wetland; Denaturing gradient gel electrophoresis (DGGE); Nitrifying bacteria; Nitrosomonas sp.; Shiwha lake;
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