Natural Product Sciences

  • 제16권4호
  • /
  • Pages.259-264
  • /
  • 2010
  • /
  • 1226-3907(pISSN)
  • /
  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Effects of the Essential Oil Components from Ligusticum chuanxiong on Proinflammatory Mediators of RAW264.7 Macrophage Cells

  • 투고 : 2010.10.13
  • 심사 : 2010.12.10
  • 발행 : 2010.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

키워드

참고문헌

  1. Beck, J.J. and Chou, S.C., The Structural diversity of phthalides from the apiaceae J. Nat. Prod., 70, 891-900 (2007). https://doi.org/10.1021/np0605586
  2. Chung, E.Y. and Shin, S., In Vitro anti-inflammatory effects of essential oil of Artemisia iwayomogi and its main component, vulgarone B. Nat. Prod. Sci. 15, 229-233 (2009).
  3. Du, J., Yu, Y., Ke, Y., Wang, C., Zhu, L., and Qian, Z.M., Ligustilide attenuates pain behavior induced by acetic acid or formalin. J. Ethnopharmacol. 112, 211-214 (2007). https://doi.org/10.1016/j.jep.2007.02.007
  4. Jeong, J.B., Ju, S.Y., Park, J.H., Lee, J.R., Yun, K.W., Kwon, S.T., Lim, J.- H., Chung, G.Y., and Jeong, H.J. Antioxidant activity in essential oils of Cnidium officinale makino and Ligusticum chuanxiong Hort and their inhibitory effects on DNA damage and apoptosis induced by ultraviolet B in mammalian cell. Cancer Epidemiol. 33, 41-46 (2009). https://doi.org/10.1016/j.canep.2009.04.010
  5. Liu, L., Ning Z.Q., Shan S., Zhang, K, Deng, T., Lu, X.P., and Cheng, Y.Y., Phthalide lactones from Ligusticum chuanxiong inhibit lipopolysaccharide induced TNF-$\alpha$ production and TNF-$\alpha$-mediated NF-$\hat{e}$B activation. Planta Med. 71, 808-813 (2005). https://doi.org/10.1055/s-2005-871231
  6. Packer, L., Ong, C.N., and Halliwell, B., Herbal and traditional medicine, Marcel Dekker, New York, pp. 247-254 (2004).
  7. Ryu, J.-H., Ahn, H., Kim, J.Y., and Kim, Y.-K., Inhibitory activity of plant extracts on nitric oxide synthesis in LPS-activated macrophages. Phytother. Res. 17, 485-489 (2003). https://doi.org/10.1002/ptr.1180
  8. Sim, Y. and Shin, S., Combinatorial anti-Trichophyton effects of Ligusticum chuanxiong essential oil components with antibiotics. Arch. Pharm. Res. 31, 497-502 (2008). https://doi.org/10.1007/s12272-001-1184-7
  9. Sim, Y. and Shin, S., Anti-Aspergillus activities of the Ligusticum chuanxiong essential oil alone and in combination with antibiotics. Nat. Prod. Sci. 16, 175-179 (2010).
  10. Su, S., Hua, Y., Duan, J.-A., Shang, E., Tang, Y., Bao, X., Lu, Y., and Ding, A., Hypothesis of active components in volatile oil from a Chinese herb formulation, 'Shao-Fu-Zhu-Yu decoction', using GC-MS and chemometrics., J. Sep. Sci. 31, 1085-1091 (2008). https://doi.org/10.1002/jssc.200700492
  11. Tewtrakul, S., Wattanapiromsakul, C., and Mahabusarakam, W., Effects of compounds from Garcinia mangostana on inflammatory mediators in RAW264.7 macrophage cells. J. Ethnopharmacol. 121, 379-382 (2009). https://doi.org/10.1016/j.jep.2008.11.007
  12. Wang. J., Du, J., Wang, Y., Kuang, X., and Wang, C., Z-ligustilide attenuates lipopolysaccharide-induced proinflammatory response via inhibiting NF-$\kappa$B pathway in primary rat microglia. Acta Pharmacol. Sin. 31, 791-797 (2010). https://doi.org/10.1038/aps.2010.71
  13. Won, J.H., Im, H.-T., Kim, Y.-H., Yun, K.I., Park, H.-J., Choi, J.W., and Lee, K.-T., Anti-inflammatory effect of buddlejasaponin IV through the inhibition of iNOS and COX-2 expression in RAW264.7 macrophages via the NF$\kappa$B inactivation. Brit. J. Pharmacol. 148, 216-225 (2006). https://doi.org/10.1038/sj.bjp.0706718
  14. Zhang, C., Qi, M., Shao, Q., Zhou, S., and Fu, R., Analysis of the volatile compounds in Ligusticum chuanxiong Hort. using HS-SPME-GC-MS. J. Pharmaceut. Biomed., 44, 464-470 (2007). https://doi.org/10.1016/j.jpba.2007.01.024
  15. Zhao, F., Wang, L., and Liu, K,. In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. J. Ethnopharmacol. 122, 457-462 (2009). https://doi.org/10.1016/j.jep.2009.01.038
  16. Zschocke, S., Klaiber, I., Bauer, R., and Vogler, B., HPLC-coupled spectroscopic techniques (UV, MS, NMR) for the structure elucidation of phthalides in Ligusticum chuanxiong. Molecular Diversity, 9, 33-39 (2005). https://doi.org/10.1007/s11030-005-1305-y