Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Generation of Antagonistic RNA Aptamers Specific to Proinflammatory Cytokine Interleukin-32

  • Kim, Se-Ho (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Jung-Hee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Yoon, Su-Jin (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Keun-Sik (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Yoon, Moon-Young (Department of Chemistry, Hanyang University) ;
  • Yoon, Do-Young (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2009.09.09
  • Accepted : 2010.09.24
  • Published : 2010.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

Interleukin 32 (IL-32) is a recently identified cytokine that induces major proinflammatory cytokines such as $TNF{\alpha}$ and IL-$1{\beta}$, which play an important role in chronic inflammatory diseases. To antagonize the biological function of IL-32 in cells, we generated RNA aptamers that could bind specifically to IL-32 protein. The highest affinity aptamer, AC3-3, successfully antagonized IL-32 by abolishing the induction of $TNF{\alpha}$ in the human lung carcinoma cells expressing IL-32. This aptamer could be used as a potent and selective antagonist against IL-32 to further elucidate the roles of IL-32 in chronic inflammatory diseases, as well as a therapeutic agent.

Keywords

References

  1. Dahl, C. A.; Schall, R. P.; He, H. L.; Cairns, J. S. J. Immunol. 1992, 148, 597.
  2. Kim, S. H.; Han, S. Y.; Azam, T.; Yoon, D. Y.; Dinarello, C. A. Immunity 2005, 22, 131.
  3. Kobayashi, H.; Lin, P. C. Cytokine 2009, 46, 351. https://doi.org/10.1016/j.cyto.2009.03.007
  4. Dinarello, C. A.; Kim, S. H. Ann Rheum Dis 2006, 65 Suppl 3, iii61. https://doi.org/10.1136/ard.2006.058511
  5. Bombardieri, M.; McInnes, I. B.; Pitzalis, C. Expert Opin Biol Ther 2007, 7, 31. https://doi.org/10.1517/14712598.7.1.31
  6. Netea, M. G.; Azam, T.; Ferwerda, G.; Girardin, S. E.; Walsh, M. et al. Proc. Natl. Acad. Sci. USA 2005, 102, 16309. https://doi.org/10.1073/pnas.0508237102
  7. Netea, M. G.; Lewis, E. C.; Azam, T.; Joosten, L. A.; Jaekal, J. et al. Proc. Natl. Acad. Sci. USA 2008, 105, 3515. https://doi.org/10.1073/pnas.0712381105
  8. Goda, C.; Kanaji, T.; Kanaji, S.; Tanaka, G.; Arima, K. et al. Int. Immunol. 2006, 18, 233. https://doi.org/10.1093/intimm/dxh339
  9. Novick, D.; Rubinstein, M.; Azam, T.; Rabinkov, A.; Dinarello, C. A. et al. Proc. Natl. Acad. Sci. USA 2006, 103, 3316. https://doi.org/10.1073/pnas.0511206103
  10. Shoda, H.; Fujio, K.; Yamaguchi, Y.; Okamoto, A.; Sawada, T. et al. Arthritis. Res. Ther. 2006, 8, R166. https://doi.org/10.1186/ar2074
  11. Joosten, L. A.; Netea, M. G.; Kim, S. H.; Yoon, D. Y.; Oppers-Walgreen, B. et al. Proc. Natl. Acad. Sci. USA 2006, 103, 3298. https://doi.org/10.1073/pnas.0511233103
  12. Kim, K. H.; Shim, J. H.; Seo, E. H.; Cho, M. C.; Kang, J. W. et al. J. Immunol. Methods 2008, 333, 38. https://doi.org/10.1016/j.jim.2007.12.017
  13. Vakkila, J.; Lotze, M. T. Nat. Rev. Immunol. 2004, 4, 641. https://doi.org/10.1038/nri1415
  14. Nishida, A.; Andoh, A.; Inatomi, O.; Fujiyama, Y. J. Biol. Chem. 2009, 284, 17868. https://doi.org/10.1074/jbc.M900368200
  15. Hong, J.; Bae, S.; Kang, Y.; Yoon, D.; Bai, X. et al. Cytokine 2010, 49, 171. https://doi.org/10.1016/j.cyto.2009.10.003
  16. Scott, D. L.; Kingsley, G. H. N. Engl. J. Med. 2006, 355, 704. https://doi.org/10.1056/NEJMct055183
  17. Dinarello, C. A. J. Exp. Med 2005, 201, 1355. https://doi.org/10.1084/jem.20050640
  18. Tuerk, C.; Gold, L. Science 1990, 249, 505. https://doi.org/10.1126/science.2200121
  19. Ellington, A. D.; Szostak, J. W. Nature 1990, 346, 818. https://doi.org/10.1038/346818a0
  20. Jayasena, S. D. Clin. Chem. 1999, 45, 1628.
  21. Rhodes, A.; Deakin, A.; Spaull, J.; Coomber, B.; Aitken, A. et al. J. Biol. Chem. 2000, 275, 28555. https://doi.org/10.1074/jbc.M002981200
  22. Seo, E. H.; Kang, J.; Kim, K. H.; Cho, M. C.; Lee, S. et al. J. Microbiol. Biotechnol. 2008, 18, 1606.
  23. Jang, K. J.; Lee, N. R.; Yeo, W. S.; Jeong, Y. J.; Kim, D. E. Biochem. Biophys. Res. Commun. 2008, 366, 738. https://doi.org/10.1016/j.bbrc.2007.12.020
  24. Zuker, M. Methods Enzymol 1989, 180, 262. https://doi.org/10.1016/0076-6879(89)80106-5
  25. Fukuda, K.; Vishnuvardhan, D.; Sekiya, S.; Hwang, J.; Kakiuchi, N. et al. Eur. J. Biochem. 2000, 267, 3685. https://doi.org/10.1046/j.1432-1327.2000.01400.x
  26. Li, W.; Yang, F.; Liu, Y.; Gong, R.; Liu, L. et al. Eur. J. Immunol. 2009, 39, 1019. https://doi.org/10.1002/eji.200838885

Cited by

  1. Comparison of Drug Delivery Efficiency between Doxorubicin Intercalated in RNA Aptamer and One Encapsulated in RNA Aptamer-Conjugated Liposome vol.36, pp.10, 2015, https://doi.org/10.1002/bkcs.10480
  2. Aptamers for Proteins Associated with Rheumatic Diseases: Progress, Challenges, and Prospects of Diagnostic and Therapeutic Applications vol.8, pp.11, 2020, https://doi.org/10.3390/biomedicines8110527