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http://dx.doi.org/10.5012/bkcs.2010.31.12.3561

Generation of Antagonistic RNA Aptamers Specific to Proinflammatory Cytokine Interleukin-32  

Kim, Se-Ho (Department of Bioscience and Biotechnology, Konkuk University)
Kim, Jung-Hee (Department of Bioscience and Biotechnology, Konkuk University)
Yoon, Su-Jin (Department of Bioscience and Biotechnology, Konkuk University)
Kim, Keun-Sik (Department of Bioscience and Biotechnology, Konkuk University)
Yoon, Moon-Young (Department of Chemistry, Hanyang University)
Yoon, Do-Young (Department of Bioscience and Biotechnology, Konkuk University)
Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
Publication Information
Bulletin of the Korean Chemical Society / v.31, no.12, 2010 , pp. 3561-3566 More about this Journal
Abstract
Interleukin 32 (IL-32) is a recently identified cytokine that induces major proinflammatory cytokines such as $TNF{\alpha}$ and IL-$1{\beta}$, which play an important role in chronic inflammatory diseases. To antagonize the biological function of IL-32 in cells, we generated RNA aptamers that could bind specifically to IL-32 protein. The highest affinity aptamer, AC3-3, successfully antagonized IL-32 by abolishing the induction of $TNF{\alpha}$ in the human lung carcinoma cells expressing IL-32. This aptamer could be used as a potent and selective antagonist against IL-32 to further elucidate the roles of IL-32 in chronic inflammatory diseases, as well as a therapeutic agent.
Keywords
Interleukin-32; Proinflammatory cytokine; RNA aptamer; $TNF{\alpha}$;
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