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Component Analysis and Antioxidant Activity of Kalopanax pictus Leaf

개두릅의 함유성분 분석과 항산화 활성

  • 신언환 (울산과학대학 호텔조리과)
  • Received : 2010.07.20
  • Accepted : 2010.07.30
  • Published : 2010.11.30

Abstract

The purpose of this study is to determine the possibility of using Kalopanax pictus leaf as natural health food source. The contents of proximate and antioxidative nutrients of Kalopanax pictus leaf were measured. The contents of carbohydrate, crude protein, crude lipid and ash were 41.42%, 45.23%, 3.29% and 10.07%, respectively. The calories of Kalopanax pictus leaf was 401.52 kcal/100 g and total dietary fiber was 30.37%. The percentages of water soluble dietary fiber to insoluble dietary fiber were 9.16% and 21.21%, respectively. The protein contained a total of 18 different kinds of amino acids. The contents of essential and non-essential amino acids were 10.51 g/100 g and 17.69 g/100 g. The K was the largest mineral followed by P, Ca, and Mg, which means Kalopanax pictus leaf is alkali material. The contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 1.56 g/100 g, 0.11 g/100 g and 1.84 g/100 g, respectively. The antioxidant activity of 70% ethanol extract and fractions of the Kalopanax pictus leaf has been determined by the scavenging of the stable radical DPPH; the result showed that the ethyl acetate fraction was the most active, as the amount required for 50% reduction of DPPH after 30 mins ($RC_{50}$) was $105.7\;{\mu}g$, followed by 70% ethanol extract ($247.3\;{\mu}g$), hexane fraction ($120.7\;{\mu}g$), chloroform ($107.3\;{\mu}g$), butanol fraction ($110.1\;{\mu}g$) and aqueous fraction ($491.9\;{\mu}g$).

개두릅의 일반성분 및 아미노산, 무기질, 지방산 함량과 항산화 효과에 대해서 조사하였다. 식품영양학적 접근에서의 개두릅의 일반성분은 건량기준으로 당질 41.42%, 조단백질 45.23%, 조지방 3.29% 및 조회분 10.07%이었고 개두릅 100 g의 함유 열량은 401.52 kcal로 분석되었으며, 총 식이섬 유소 함량은 건량기준으로 30.37%이었고 수용성 및 불용성 식이섬유소 함량은 각각 9.16%, 21.21%로 나타났다. 또한, 총 18종의 아미노산으로 구성되었으며 필수아미노산과 비필수아미노산 함량은 각각 10.51 g, 17.69 g이었고, 무기질 중 칼륨의 함유량이 가장 높았고 그 다음이 인, 칼슘, 마그네슘 순으로 나타나 알칼리성 재료임을 알 수 있었으며, 지방산 함량의 경우 총 포화지방산 1.56 g, 단일불포화지방산 0.11 g 및 다가불포화지방산 1.84 g로 구성되어 있었다. 개두릅 70% 에탄올, 헥산, 클로로포름, 에틸아세테이트, 부탄올과 물 추출물에서 DPPH 라디칼 소거능의 항산화 효과 결과 는 $RC_{50}$값이 247.3, 120.7, 107.3, 105.7, 110.1, $491.9\;{\mu}g$으로 나타나, 에틸아세테이트 분획물에서 가장 높은 항산화 효과를 나타내었다.

Keywords

References

  1. Yim JE, Choue RW, Kim YS. 1998. Effect of dietary counseling and HMG CoA reductase inhibitor treatment on serum lipid levels in hyperlipidemic patients. Korean J Lipidol 8: 61-76.
  2. Moon SJ. 1996. Korean disease pattern and nutrition. Korean J Nutr 29: 381-383.
  3. Han SM. 2001. Studies on the functional components and cooking aptitude for medicinal tea of Chrysanthemum indicum L. MS Thesis. Sejong University, Seoul, Korea.
  4. Technology Roadmap in Korea. 2003. National Technology Roadmap. Aiming at Bio-healthtopia. STEPI, Seoul, Korea. p 123-154.
  5. Han HK, Lim SJ. 1998. Effect of fractions from methanol extract of Commelina ommuris on blood glucose level and energy metabolism in streptozotocin-induced diabetic rats. Korean J Soc Food Sci 14: 577-583.
  6. Hong JS, Kim YH, Lee KR, Kim MK, Cho CI, Park KH, Choi YH, Lee JB. 1998. Composition of organic acid, fatty acid in Pleurotus ostreatus, Lentinus edodes and Agaricus bisporus. Korean J Food Sci Technol 20: 100-106.
  7. Lee GD, Chang HG, Kim HK. 1997. Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J Food Sci Technol 29: 432-436.
  8. Park SH, Han JH. 2003. The effects of uncooked powdered food on nutrient intake, serum lipid level, dietary behavior and health index in healthy women. J Nutr 36: 49-63.
  9. Choi MS, Do DH, Choi DJ. 2002. The effect of mixing beverage with Aralia continentatis Kitagawa root on blood pressure and blood constituents of the diabetic and hypertensive elderly. Korean J Food Nutr 15: 165-172.
  10. Cha WS, Kim CK, Kim JS. 2002. On the development of functional health beverages using Citrus reticulate, Ostrea gigas. Korean J Biotechnol Bioeng 17: 503-507.
  11. Kim JH, Park JH, Park SD, Choi SY, Seong JH, Moon KD. 2002. Preparation and antioxidant activity of health drink with extract powders from safflower seed. Korean J Food Sci Technol 34: 617-624.
  12. Han H, Song YJ, Park SH. 2004. Development of drink from composition with medicinal plants and evaluation of its physiological function in aorta relaxation. Korean J Oriental Physiology & Pathology 18: 1078-1082.
  13. Sano K, Sanada S, Ida Y, Shoji J. 1991. Studies on the constituents of the stem bark of Kalopanax pictus Nakai. Chem Pharm Bull 39: 865-870. https://doi.org/10.1248/cpb.39.865
  14. Shao CJ, Nakai R, Ohtani K, Xu JD, Tanaka O. 1989. Saponines from leaves of Kalopanax septemlobus (Thunb.) Koidz, structures of kalopanaxsaponins La, Lb and Lc. Chem Pharm Bull 37: 3251-3252. https://doi.org/10.1248/cpb.37.3251
  15. Choi JW, Huh K, Kim SH, Lee KT, Park HJ, Han YN. 2002. Antinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals. J Ethnopharmacol 79: 199-204. https://doi.org/10.1016/S0378-8741(01)00383-X
  16. Lee KT, Sohn IC, Park HJ, Kim DW, Jung GO, Park KY. 2002. Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides isolated from the stem bark of Kalopanax pictus. Planta Med 66: 329-332. https://doi.org/10.1055/s-2000-8539
  17. Park HJ, Kwon SH, Lee JH, Lee KH, Miyamoto K, Lee KT. 2001. Kalopanaxsaponin A is a basic saponin structure for the anti-tumor activity of hederagenin monodesmosides. Planta Med 67: 118-121. https://doi.org/10.1055/s-2001-11516
  18. AOAC. 1990a. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 788.
  19. Korea Food and Drug Administration. 2002. Food Standard Codex. Korean Foods Industry Association, Seoul, Korea. p 301-301.
  20. AOAC. 1995a. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. Chapter 45, p 70.
  21. AOAC. 1995b. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. Chapter 32, p 5.
  22. Waters Associates. 1983. Official method of amino acid analysis. In Amino Acid System of Operators Manual of the Waters Associates. Milford, MA, USA. p 37.
  23. AOAC. 1984a. Official Methods of Analysis. 14th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 878.
  24. Folch J, Lees M, Slane SGH. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
  25. Kim SY, Ryu CH. 1995. Studies on the nutritional components of purple sweet potato (Ipomoea batatas). Korean J Food Technol 27: 819-825.
  26. Choi JS, Park JH, Kim HG, Young HS, Mun SI. 1993. Screening for antioxidant activity of plants and marine algae and its active principles from Prunus daviana. Kor J Phamacology 24: 299-303.
  27. SAS. 1987. SAS/STAT guide for personal computer. version 6th ed. SAS Institute Inc., Cary, NC, USA. p 60.
  28. Hwang JK. 1996. Phytochemical properties of dietary fiber. J Korean Soc Food Nutr 25: 715-719.
  29. Han GJ, Shin DS, Jang MS. 2008. A study of the nutritional composition of Aralica continentalis Kitagawa and Aralica continentalis Kitagaea leaf. Korean J Food Sci Technol 40: 680-685.
  30. Shin KK, Yang CB, Park H. 1992. Studies on lipid and fatty acid composition of Korea perilla leaves (Penilla frutescens var. japonica HARA). Korean J Food Sci Technol 24: 610-615.
  31. Lee BY, Hwang JB. 1998. Some components analysis for Chinese water chestnut processing. Korean J Food Sci Technol 30: 717-720.
  32. Hwang JB, Yang MO, Shin HK. 1997. Survey for approximate composition and mineral content of medicinal herb. Korean J Food Sci Technol 29: 671-679.
  33. Hwang JB, Yang MO, Shin HK. 1998. Survey for amino acid of medicinal herb. Korean J Food Sci Technol 30: 35-41.
  34. Park YK. 2001. Studies on the effects of Puerariae Flos, Curcumae Radix and Sophorae Radix on the antioxidation. Kor J Herbology 16: 41-53.
  35. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 27: 978-984.

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