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Analysis of Chemical Composition and Antioxidant Activity of Codonopsis lanceolata Skin

더덕껍질의 일반성분 분석과 항산화 활성

  • Kim, Na-Young (Dept. of Hotel Culinary Arts, Songho College) ;
  • Chae, Hyun-Seok (Dept. of Hotel Culinary Arts, Songho College) ;
  • Lee, In-Sook (Hoengseong-Gun Agricultural Technology and Extension Center) ;
  • Kim, Dong-Soo (Hotel Tourism Department (Food Service Culinary Major), Kwan Dong University) ;
  • Seo, Kang-Tae (Professor in Foodservice Industry Division, Baeseok Culture University) ;
  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym College)
  • 김나영 (송호대학 호텔외식조리과) ;
  • 채현석 (송호대학 호텔외식조리과) ;
  • 이인숙 (횡성군농업기술센터) ;
  • 김동수 (관동대학교 호텔관광학부 외식조리) ;
  • 서강태 (백석문화대학 외식산업학부) ;
  • 박성진 (한림성심대학 관광외식조리과)
  • Received : 2010.07.19
  • Accepted : 2010.08.22
  • Published : 2010.11.30

Abstract

The purpose of this study is to determine the possibility of Codonopsis lanceolata skin as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of C. lanceolata skin were measured. On a dry weight basis the contents of carbohydrate, crude protein, crude lipid and ash are 24.74, 2.73, 2.96 and 4.84%, and the calories of skin was 266.00 kcal/100 g and total dietary fiber was 64.73%. The contents of essential and non-essential amino acids were 633.40 and 870.72 mg/100 g wet weight basis. The K was the largest mineral followed by Ca, Mg, and P, suggesting that C. lanceolata skin is alkali material. The EDA of water extract from C. lanceolata skin was 18.28~79.30%, and the activity was dependent on the sample concentration. Total phenolic and flavonoids contents of water extract from C. lanceolata skin were estimated as 24.65 and $6.19\;{\mu}g/g$. The C. lanceolata skin extract showed the highest reducing power (3.5) at the concentration of 25 mg/mL. Based on the above results, we deemed that the C. lanceolata skin might have potential antioxidant activities. The general nutrients and antioxidant bioactive materials in C. lanceolata skin were also potential materials for good health food.

본 연구는 더덕껍질의 기능성식품 및 화장품 소재로서의 이용가능성을 조사하기 위해서 더덕껍질의 영양성분 분석을 통한 식품영양학적 접근, 생리활성 기능을 기대할 수 있는 관련 물질 함량을 분석하였다. 식품영양학적 접근에서의 더덕껍질의 일반성분은 건량기준으로 탄수화물 24.74%, 조단백질 2.73%, 조지방 2.96% 및 조회분 4.84%이었고 더덕껍질 100 g의 함유 열량은 266.00 kcal로 분석되었으며, 총 식이섬유소 함량은 건량기준으로 64.73%로 나타났다. 또한, 필수아미노산과 비필수아미노산 함량은 각각 633.40과 870.72 mg/100 g wet weight basis이었고, 무기질 중 칼륨의 함유량이 가장 높았고 그 다음이 칼슘, 마그네슘, 인순으로 나타나 알칼리성 재료임을 알 수 있었으며, 지방산 함량의 경우 불포화지방산의 함량이 높은 것으로 나타났다. 유리당의 경우 fructose의 함량이 전체 유리당의 약 72%를 차지하고 있는 것으로 나타났다. 더덕껍질 물추출물의 총 페놀화합물 함량은 $24.65\;{\mu}g/g$, 플라보노이드 함량은 $6.19\;{\mu}g/g$으로 분석되었으며, DPPH 소거 활성을 농도별로 측정하여 비교 한 결과 $500\;{\mu}g/mL$ 농도에서 79.30%로 높은 활성을 보였다. 또한, 환원력의 경우에는 더덕껍질 추출물이 25 mg/mL의 농도에서 3.5로 높은 환원력을 보였다. 이상의 결과로부터 더덕껍질 물추출물은 항산화능이 있음을 확인할 수 있었다. 따라서 더덕껍질을 식품 내 첨가물로서, 혹은 다른 약용으로의 활용가능성을 제시하고 있다.

Keywords

References

  1. Yim JE, Choue RW, Kim YS. 1998. Effect of dietary counseling and HMG CoA reductase inhibitor treatment on serum lipid levels in hyperlipidemic patients. Korean J Lipidology 8: 61-76.
  2. Moon SJ. 1996. Korean disease pattern and nutrition. Korean J Nutr 29: 381-383.
  3. Han SM. 2001. Studies on the functional components and cooking aptitude for medicinal tea of Chrysanthemum indicum L. MS Thesis. Sejong University, Seoul, Korea.
  4. Technology Roadmap in Korea. 2003. National Technology Roadmap. Aiming at Bio-healthtopia. STEPI, Seoul, Korea. p 23-154.
  5. Han HK, Lim SJ. 1998. Effect of fractions from methanol extract of Commelina ommuris on blood glucose level and energy metabolism in streptozotocin-induced diabetic rats. Korean J Soc Food Sci 14: 577-583.
  6. Hong JS, Kim YH, Lee KR, Kim MK, Cho CI, Park KH, Choi YH, Lee JB. 1998. Composition of organic acid, fatty acid in Pleurotus ostreatus, Lentinus edodes & Agaricus bisporus. Korean J Food Sci Technol 20: 100-106.
  7. Lee GD, Chang HG, Kim HK. 1997. Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J Food Sci Technol 29: 432-436.
  8. Park SH, Han JH. 2003. The effects of uncooked powdered food on nutrient intake, serum lipid level, dietary behavior and health index in healthy women. J Nutr 36: 49-63.
  9. Choi MS, Do DH, Choi DJ. 2002. The effect of mixing beverage with Aralia continentalis Kitagawa root on blood pressure and blood constituents of the diabetic and hypertensive elderly. Korean J Food & Nutr 15: 165-172.
  10. Cha WS, Kim CK, Kim JS. 2002. On the development of functional health beverages using Citrus reticulate, Ostrea glgas. Korean J Biotechnol Bioeng 17: 503-507.
  11. Kim JH, Park JH, Park SD, Choi SY, Seong JH, Moon KD. 2002. Preparation and antioxidant activity of health drink with extract powders from safflower seed. Korean J Food Sci Technol 34: 617-624.
  12. Han H, Song YJ, Park SH. 2004. Development of drink from composition with medicinal plants and evaluation of its physiological function in aorta relaxation. Korean J Oriental Physiology & Pathology 18: 1078-1082.
  13. Kim PJ. 2002. Study on the diet according to the sasang constitution. MS Thesis. Dong Eui University, Busan, Korea.
  14. Seo MW, Jeong SI, Shin CG, Ju YS. 2003. The morphological standard and isolation and structure elucidation of radical scavengers from Chrysanthemum indicum L. Korean J Herbology 18: 133-144.
  15. Kim CH, Chung MH. 1975. Pharmacognostical studies on Condopsis lanceolata. Natural Product Sciences 6: 43-47.
  16. Maeng YS, Park HK. 1991. Antioxidant activity of ethanol extract from Dodok (Codonopsis lanceolata). Korean J Food Sci Technol 23: 311-316.
  17. Kim SY, Kim HS, Su IS, Yi HS, Kim HS, Chung SY. 1993. Effects of the feeding Platycodon grandiflorum and Codonopsis lanceolata on the lipid components of serum and liver in rats. J Korean Soc Food Nutr 22: 517-523.
  18. Han EG, Sung IS, Moon HG, Cho SY. 1998. Effect of Codonopsis lanceolata water extract on the levels of lipid in rats fed high fat diet. J Korean Soc Food Nutr 27: 940-9443.
  19. Hong WS, Lee JS, Kim EJ, Choi YS. 2006. A study on the perception of Codonopsis lancedata Dishes and development of Codonopsis lancedata Dishes. Korean J Food Cookery Sci 22: 181-192.
  20. Kang YH. 2009. Phenolic compounds and antioxidant activity in cell wall materials from Deodeok (Codonopsis lanceolata). Korean J Food Technol 41: 345-349.
  21. AOAC. 1990a. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 788.
  22. AOAC. 1995a. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. Chapter 45, p 70.
  23. Waters Associates. 1983. Official methods of analysis. In Amino acid system of operators manual of the Waters Associates. Milford, MA, USA. p 37.
  24. AOAC. 1984a. Official Methods of Analysis. 14th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 878.
  25. Folch J, Lees M, Slane SGH. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
  26. Bligh EG, Dyer WJ. 1959. A rapid methods of total lipid extraction and purification. Can J Biochem Physil 37: 911-917. https://doi.org/10.1139/o59-099
  27. Richmond ML, Brandao SCC, Gray JI, Markakis P, Stine CM. 1981. Analysis of simple sugar and sorbitol in fruit by HPLC. J Agric Food Chem 29: 4-7. https://doi.org/10.1021/jf00103a002
  28. Lee HH, Lee SY. 2008. Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean J Medicinal Crop Sci 16: 79-85.
  29. Gutfinger T. 1981. Polyphenols in olive oils. JAOCS 58: 966-967. https://doi.org/10.1007/BF02659771
  30. Moreno MIN, Isla MIN, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several region of Argentiana. J Enthropharmacology 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  31. Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. J apanese J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  32. Won HR, Oh HS. 2007. Antioxidative activity and lipid composition from different part and supplement of Codonopsis lanceolata in rat. J Korean Soc Food Sci Nutr 36: 1128-1133. https://doi.org/10.3746/jkfn.2007.36.9.1128
  33. Kim SH. 2009. Cholesterol lowering activities of Codonopsis lanceolata and Platycodon grandiflorum extracts. PhD Dissertation. Kangwon National University, Chuncheon, Korea. p 29-30.
  34. Duval B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed andise root extract. J Food Biochem 25: 361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  35. Choi KS, Lee HY. 1999. Characteristics of useful components in the leaves of Baechohyang (Agastache rugosa, O. Kuntze). J Korean Soc Food Sci Nutr 28: 326-332.
  36. Middleton EJ, Kandaswami C. 1994. Potential health promoting properties of citrus flavonoids. Food Technol 48: 115.
  37. Kim SH, Chung MJ, Jang HD, Han SS. 2010. Antioxidative activity of Codonopsis lanceolata extract in vitro and in vivo. J Korean Soc Food Sci Nutr 39: 193-202. https://doi.org/10.3746/jkfn.2010.39.2.193
  38. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 624-630.
  39. Yim MH, Hong TG, Lee JH. 2006. Antioxidant and antimicrobial activity of fermentation and ethanol extracts of pine needles (Pinus densiflora). Food Sci Biotechnol 15: 582-588.

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