Effect of Storage Conditions on the Quality of Green Tea Beverage

저장 조건이 녹차 음료의 품질에 미치는 영향

  • Lee, Jung-Min (Dept. of Food Science and Biotechnology, Kyungnam Univ.) ;
  • Lim, Sang-Wook (Dept. of Food Science and Biotechnology, Kyungnam Univ.) ;
  • Cho, Sung-Hwan (Div. of Applied Life Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Choi, Sung-Gil (Div. of Applied Life Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Heo, Ho-Jin (Div. of Applied Life Science(Insti. of Agric. and Life Sci.), Gyeongsang National Univ.) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam Univ.)
  • 이정민 (경남대학교 식품생명학과) ;
  • 임상욱 (경남대학교 식품생명학과) ;
  • 조성환 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 이승철 (경남대학교 식품생명학과)
  • Received : 2009.04.17
  • Accepted : 2009.06.20
  • Published : 2009.06.30

Abstract

Green tea was prepared by soaking 1.5 g of green tea leaves into 100 mL of distilled water at $75^{\circ}C$ for 5 min. The green tea was stored at three different conditions - (A) green tea was stored at not-artificially excluding natural light condition, at natural air condition, and without addition of vitamin C; (B) green tea was stored at artificially excluding natural light condition by wrapping a vial with aluminium foil, at nitrogen filling up condition, and with addition of 30 mg/100 mL of vitamin C; and (C) green tea was stored at artificially excluding natural light condition by wrapping a vial with aluminium foil, at nitrogen filling up condition, and without addition of vitamin C. After 28 days of storage at $4^{\circ}C$, the chemical quality of the green tea was evaluated. Total phenolic contents of (A), (B), and (C) green tea decreased to 71.50, 73.88 and 75.07%, respectively, after storing for 28 days compared to those of beginning state. DPPH radical scavenging activities of (A), (B), and (C) green tea were 87.87, 92.93 and 88.39%, respectively. Epigallocatechin gallate (EGCG), the main active compounds of green tea, contents of (A), (B), and (C) green tea were 130.61, 136.47 and 4.34%, respectively. The results indicated that light, air condition, and vitamin C were significantly important to the chemical quality of green tea during storage.

녹차 잎 1.5 g에 증류수 100 mL을 가하여 $75^{\circ}C$에서 5분 동안 추출하여 녹차 음료를 제조하였다. 저장 조건을 달리하여 A 녹차 음료 (빛을 인위적으로 차단하지 않음, 자연 상태의 대기 조건, 비타민 C 무첨가), B 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 30 mg/100 mL 첨가), C 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 무첨가)로 구분하여 $4^{\circ}C$에서 28일간 저장한 후, 유용 성분을 분석하였다. 총페놀 함량의 경우 저장 초기에 비하여 저장 28일 후 A 녹차 음료는 71.50%, B 녹차 음료는 73.88%, C 녹차 음료는 75.07%로 감소하였다. DPPH 라디컬 소거능은 저장 초기에 비하여 A 녹차 음료는 87.87%, B 녹차 음료는 92.93%, C 녹차 음료는 88.39%이었다. 녹차의 유용 카테킨인 epigallocatechin gallate (EGCG)는 28일간 저장 후에 A 녹차 음료는 130.61%, B 녹차 음료는 136.47%, C 녹차 음료는 4.34%이었다. 이상의 결과는 녹차의 저장 조건이 녹차 음료의 성분에 영향을 미치며, 빛, 질소 치환, 비타민 C가 매우 중요함을 시사하였다.

Keywords

Acknowledgement

Supported by : 농림부

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