Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Phoma herbarum as a New Gibberellin-Producing and Plant Growth-Promoting Fungus

  • Hamayun, Muhammad (School of Applied Biosciences, Kyungpook National University) ;
  • Khan, Sumera Afzal (Centre of Biotechnology and Microbiology, University of Peshawar) ;
  • Khan, Abdul Latif (School of Applied Biosciences, Kyungpook National University) ;
  • Rehman, Gauhar (Department of Genetic Engineering, Kyungpook National University) ;
  • Sohn, Eun-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Shah, Aamer Ali (Department of Biotechnology, Quaid-i-Azam University) ;
  • Kim, Sang-Kuk (Institute for Bioresources Research, Gyongbuk Provincial Agricultural Technology Administration) ;
  • Joo, Gil-Jae (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University)
  • Published : 2009.10.31
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Abstract

Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK-2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially $GA_4$ and $GA_7$, were present in higher amounts ($GA_1$, 0.11 ng/ml; $GA_3$, 2.91 ng/ml; $GA_4$, 3.21 ng/ml; and $GA_7$, 1.4 ng/ml) in conjunction with physiologically inactive $GA_9$ (0.05 ng/ml), $GA_{12}$ (0.23 ng/ ml), $GA_{15}$ (0.42 ng/ml), $GA_{19}$ (0.53 ng/ml), and $GA_{20}$ (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence.

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References

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