Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Clitocybin D, a Novel Human Neutrophil Elastase Inhibitor from the Culture Broth of Clitocybe aurantiaca

  • Kim, Young-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryoo, In-Ja (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choo, Soo-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Xu, Guang-Hua (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang-Ku (Korea Research Institute of Bioscience and Biotechnology) ;
  • Seok, Soon-Ja (National Institute of Agricultural Science and Technology, RDA) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2009.10.31
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Abstract

Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significant HNE inhibitory activity with an $IC_{50}$ value of $17.8{\mu}M$.

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References

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