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http://dx.doi.org/10.4014/jmb.0903.03033

Clitocybin D, a Novel Human Neutrophil Elastase Inhibitor from the Culture Broth of Clitocybe aurantiaca  

Kim, Young-Hee (Korea Research Institute of Bioscience and Biotechnology)
Ryoo, In-Ja (Korea Research Institute of Bioscience and Biotechnology)
Choo, Soo-Jin (Korea Research Institute of Bioscience and Biotechnology)
Xu, Guang-Hua (Korea Research Institute of Bioscience and Biotechnology)
Lee, Sang-Ku (Korea Research Institute of Bioscience and Biotechnology)
Seok, Soon-Ja (National Institute of Agricultural Science and Technology, RDA)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.10, 2009 , pp. 1139-1141 More about this Journal
Abstract
Clitocybin D, a novel human neutrophil elastase inhibitor, was isolated from the culture broth of Clitocybe aurantiaca. This compound was purified by solvent extraction, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The compound was determined to be 4-(4,6-dihydroxy-3-methoxy-3H-isoindol-1-yl)-benzoic acid on the basis of 1D and 2D NMRs and MS spectroscopic analysis. Analysis of the human neutrophil elastase (HNE) inhibitory activity of the isolated compound revealed that it showed significant HNE inhibitory activity with an $IC_{50}$ value of $17.8{\mu}M$.
Keywords
Clitocybe aurantiaca; clitocybin D; human neutrophil elastase;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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