KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Polysaccharide Characteristics from Hot Water Extract of Aloe saponaria Callus

Aloe saponaria 캘러스의 열수 추출물 유래 다당의 특성

  • Baek, Jin-Hong (KJM Aloe R&D Center) ;
  • Kim, Myung-Uk (Department of Bioengineering and Technology, Kangwon National University) ;
  • Kang, Tae-Su (Department od Biofood Science and Biotechnology, Chungbuk Provincial College of Science & Technology) ;
  • Hur, Won (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Shin-Young (Department of Bioengineering and Technology, Kangwon National University)
  • 백진홍 ((주) 김정문 알로에 과학연구소) ;
  • 김명욱 (강원대학교 생물공학과) ;
  • 강태수 (충북과학대학 바이오식품생명과학과) ;
  • 허원 (강원대학교 생물공학과) ;
  • 이신영 (강원대학교 생물공학과)
  • Published : 2009.02.28
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Abstract

The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.

Keywords

References

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