Korean Journal of Acupuncture

  • 제26권3호
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  • Pages.69-76
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  • 2009
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  • 2287-3368(pISSN)
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  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
권호 표지

토복령(土茯笭)이 RAW264.7 대식세포주에서 산화질소 억제에 미치는 영향

Inhibitory effect of Smilacis Glabrae Rhizoma on nitric oxide production in the macrophage cell line RAW 264.7

  • 이강희 (세명대학교 한의과대학 경혈학교실) ;
  • 정준희 (세명대학교 한의과대학 경혈학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 이재혁 (세명대학교 한의과대학 한방신경정신과학교실)
  • Lee, Kang-Hee (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Jung, Jun-Hee (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Lee, Jae-Hyuk (Dept. of Oriental Neuropsychiatry, College of Oriental Medicine, Semyung University)
  • 발행 : 2009.09.27
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초록

Objectives : 본 연구의 목적은 제습, 해독, 통리관절등의 효능이 있는 토복령이 RAW264.7 대식세포주에서 lipopolysaccharide(LPS)로 처치하여 생성되는 nitric oxide(NO)와 prostaglandin $E_2$($PGE_2$)에 억제작용이 있는지 관찰하고자 하는 것이다. Methods : 토복령이 RAW264.7 대식세포주에 세포독성이 유무를 관찰하기 위하여 농도별 MTT assay를 수행하여 세포생존율을 측정하였다. 또한 LPS로 염증유발된 RAW264.7 대식세포주에서 토복령의 농도별 처치가 NO 및 $PGE_2$ 생성억제에 미치는 영향을 관찰하고자 NO 및 $PGE_2$ assay를 수행하였다. Results : 토복령의 농도별 처치가 RAW264.7 대식세포주에서 세포독성을 유발하는지 MTT assay로 측정한 결과 세포독성은 관찰되지 않았으며, 토복령은 LPS처치로 인하여 증가된 NO 및 $PGE_2$ 생성을 통계학적으로 유의하게 감소시킴을 관찰하였다. Conclusions : 본 연구를 통하여 토복령은 RAW264.7 대식세포주에서 LPS로 유도된NO 및 $PGE_2$ 생성을 효과적으로 억제시킴으로써 추후 염증질환의 치료제로서 가능성을 확인하였다.

키워드

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