Korean Journal of Acupuncture

  • 제26권3호
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  • Pages.55-68
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  • 2009
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  • 2287-3368(pISSN)
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  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
권호 표지

황금(黃芩) 약침(藥鍼)이 급성염증 백서의 면역조절능에 미치는 영향

Immunomodulatory activity of Scutellaria baicalensis Georgi Pharmacopuncture on Acute Inflammatory Rat Model

  • 양승희 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이향숙 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 이준무 (상지대학교 한의과대학 경락경혈학교실)
  • Yang, Seung-Hee (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University) ;
  • Lee, Hyang-Sook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of health Science, Sangji University) ;
  • Lee, Joon-Moo (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji University)
  • 발행 : 2009.09.27
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초록

Objectives : To investigate the anti-inflammatory effects of Scutellaria baicalensis Georgi pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+Scutellaria baicalensis Georgi pharmacopuncture at BL23 (n=6, BL23), LPS+Scutellaria baicalensis Georgi pharmacopuncture at CV12 (n=6, CV12), and LPS+Scutellaria baicalensis Georgi pharmacopuncture at GV4 (n=6, GV4). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by intraperitoneal LPS injection (5mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results : For proinflammatory cytokines, CV12 pharmacopuncture group was significantly different compared with the control group in plasma IL-$1{\beta}$, IL-6, TNF-$\alpha$, and IL-10 5 h after LPS injection (P<0.05). For plasma IL-$1{\beta}$ and IL-6, CV12 pharmacopuncture group also showed significant difference at 2 h compared with the control (P<0.05). GV4 pharmacopuncture group was significantly different compared with the control at 5 h in plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ and at 2 h in IL-10 (P<0.05). Liver cytokines were analyzed at 5 h after LPS injection; only CV12 pharmacopuncture group showed significant difference in IL-$1{\beta}$ (P<0.05) and others including IL-6, TNF-$\alpha$, and IL-10 had no difference compared with the control group. CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). Plasma NO3-/NO2- and intracellular adhesion molecule-1 of CV12 pharmacopuncture group were significantly lower than that of the control group (P<0.05). In the plasma concentration of prostaglandin E2, all 3 pharmacopuncture groups had significantly lower values than that of the control group (P<0.05), but there was no difference among pharmacopucnture groups. Monocyte chemoattractant protein-1 and cytokine-induced neutorphil chemoattractant-1 in peritoneal lavage fluid was significantly decreased in CV12 pharmacopuncture group compared with the control group (P<0.05). Conclusions : These results indicate that Scutellaria baicalensis Georgi pharmacopuncture at CV12 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

키워드

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