Korean Journal of Acupuncture

  • 제26권3호
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  • Pages.43-54
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  • 2009
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  • 2287-3368(pISSN)
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  • 2287-3376(eISSN)
경락경혈학회 (Society for Meridian and Acupoint)
권호 표지

하고초 약침이 LPS로 유발된 급성염증 백서 모델에 미치는 영향

Effects of Prunella vulgaris Pharmacopuncture on Lipopolysaccharide-Induced Acute Inflammatory Rat Model

  • 이종욱 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이향숙 (상지대학교 한의과대학 경락경혈학교실) ;
  • 이은 (상지대학교 보건과학대학 제약공학과) ;
  • 이준무 (상지대학교 한의과대학 경락경혈학교실)
  • Lee, Jong-Wook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university) ;
  • Lee, Hyang-Sook (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university) ;
  • Lee, Eun (Dept. of Pharmaceutical Engineering, College of healt Science, Sangji university) ;
  • Lee, Joon-Moo (Dept. of Meridian & Acupoint, College of Korean Medicine, Sangji university)
  • 발행 : 2009.09.27
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초록

Objectives : To investigate the anti-inflammatory effects of Prunella vulgaris pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 5 groups; normal control (n=8), LPS control (n=8), LPS+Prunella vulgaris pharmacopuncture at CV4 (CV4, n=8), LPS+Prunella vulgaris pharmacopuncture at ST36 (ST36, n=8), and LPS+Prunella vulgaris pharmacopuncture at CV12 (CV12, n=8). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5mg/kg). Proinflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-10 (IL-10), thiobarbituric acid reactive substance (TBARS) from blood and liver tissue were compared before and 5 hrs after inflammation induction. Results : In CV4 and CV12 groups, plasma IL-$1{\beta}$, IL-6 and TNF-$\alpha$ levels increased by LPS injection, significantly decreased 5 hrs after injection (p<0.05). For CV12 group, plasma IL-10 concentration significantly increased (p<0.05). Liver IL-$1{\beta}$ and IL-6 levles significantly decreased in CV4 and CV12 groups (P<0.05), while normal and LPS control groups were not significantly different in TNF-$\alpha$ and IL-10 levels. Plasma TBARS concentration was significantly decreased in CV12 group, while there was no significant difference among LPS control and pharmacopuncture groups for liver TBARS concentration. Conclusions : Based on the present findings, Prunella vulgaris pharmacopuncture at CV12 may have a potentially preventive anti-inflammatory effect in an LPS-induced inflammatory rat model.

키워드

참고문헌

  1. Male D. Cell migration and inflammation. In: Roitt I, Brostoff J, Male D. Immunology. 5th ed. London : Mosby. 1998 : 61-9.
  2. Boumpas DT, Chrousos GP, Wilder RL, Cupps TR, Balow JE. Glucocorticoid therapy for immune-mediated diseases: Basic and clinical correlates. Ann Intern Med. 1993 ; 119(12) : 1198-208. https://doi.org/10.7326/0003-4819-119-12-199312150-00007
  3. 김현제 외 편역. 한의학사전. 서울 : 성보사. 1988 : 194.
  4. 문류모. Stress에 관한 문헌적 고찰 - 현대의학을 중심으로. 동의신경정신과학회지. 1991 ; 2(1) : 38-50.
  5. 전국한의과대학 본초학교수 공편저. 본초학. 서울 : 도서출판 영림사. 1991 : 169-70.
  6. 대한약침학회학술위원회. 약침학. 서울 : 엘스비어코리아. 2008 : 6-7.
  7. WHO standard acupuncture point locations in the western pacific region. WHO Library Cataloguing in Publication Data. 2008 : 64, 221, 225.
  8. Buge JA, Austa SD. Microsomal lipid peroxidation. In: Fleicher S, Packer L. Methods in Enzymology. 1st ed. London : Academic Press. 1978 : 302-9.
  9. Rook G. Immunity to bacteria and fungi. In: Roitt I, Brostoff J, Male D. Immunology. 5th ed. London : Mosby. 1998 : 229-32.
  10. 전국한의과대학, 한의학전문대학원 경락경혈학 교재편찬위원회. 대학경락경혈학각론(상, 하). 의방출판사. 2009 : 259, 1137, 1172.
  11. 임사비나, 임형택, 박히준, 장지련, 최일환, 이석찬 외. 족삼리 침자극의 복통 억제기전연구. 대한경락경혈학회지. 2004 ; 21(2) : 69-79.
  12. 김동윤, 김경호, 김갑성, 송춘호, 안창범. 胃兪穴과 中脘穴의 蒼朮水鍼이 胃炎回復 效果에 미치는 影響. 대한침구학회지. 1993 ; 10(1) : 289-96.
  13. 고인자, 유승조, 이은방. 한국산 하고초류의 약물학적 연구(1). 생약학회지. 1986 ; 17(3) : 232-41.
  14. 이수정, 성낙주, 정혜광, 신정혜, 정영철, 서종권. 하고초 메탄올 추출물의 항산화 활성. 한국식품영양과학회지. 2008 ; 37(12) : 1535-41.
  15. 한양희. 夏枯草가 만성 비세균성 전립선염 rat의 전립선세포 조직변화 및 염증관련 cytokines 발현에 미치는 영향. 대한한의학회지. 2008 ; 29(2) : 71-80.
  16. 이윤섭. 한상원. 꿀풀과 및 댑싸리 夏枯草藥鍼이 갑상선기능항진증에 미치는 영향. 대한침구학회지. 1997 ; 14(1) : 494-502.
  17. Han EH, Choi JH, Hwang YP, Park HJ, Choi CY, Chung YC et al. Immunostimulatory activity of aqueous extract isolated from Prunella vulgaris. Food Chem Toxicol. 2009 ; 47(1) : 62-9. https://doi.org/10.1016/j.fct.2008.10.010
  18. Mathiak G, Grass G, Herzmann T, Luebke T, Zetina CC, Boehm SA et al. Capase-1-inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J Pharmacol. 2000 ; 131(3) : 383-6. https://doi.org/10.1038/sj.bjp.0703629
  19. Chamulitrat W, Blazka ME, Jordan SJ, Luster MI, Mason RP. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 1995 ; 57(24) : 2273-80. https://doi.org/10.1016/0024-3205(95)02220-D
  20. Harbrecht BG, Di Silvio M, Demetris AJ, Simmons RL, Billiar TR. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology. 1994 ; 20(4 Pt 1) : 1055-60. https://doi.org/10.1002/hep.1840200439
  21. Hamada E, Nishida T, Uchiyama Y, Nakamura J, Isahara K, Kazuo H et al. Activation of Kupffer cells and caspases-3 involved in rat hepatocyte apoptosis induced by endotoxin. J Hepatol. 1999 ; 30(5) : 807-18. https://doi.org/10.1016/S0168-8278(99)80133-0
  22. Thompson KC, Trowern A, Fowell A, Marathe M, Haycock C, Arthur MJ et al. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology. 1998 ; 28(6) : 1518-24. https://doi.org/10.1002/hep.510280611
  23. Sang H, Wallis GL, Stewart CA, Kotake Y. Expression of cytokines and activation of transcription factors in lipopolysaccharide-administered rats and their inhibition by phenyl N-tert-butylnitrone (PBN). Arch Biochem Biophys. 1999 ; 363(2) : 341-8. https://doi.org/10.1006/abbi.1998.1086
  24. Louis H, Le Moine O, Peny MO, Quertinmont E, Fokan D, Goldman M et al. Production and role of interleukin-10 in concanavalin A-induced hepatitis in mice. Hepatology. 1997 ; 25(6) : 1382-9. https://doi.org/10.1002/hep.510250614
  25. Simpson KJ, Lukacs NW, Colletti L, Strieter RM, Kunkel SL. Cytokines and the liver. J Hepatol. 1997 ; 27(6) : 1120-32. https://doi.org/10.1016/S0168-8278(97)80160-2
  26. 이은, 신주옥. 과산화지질을 급여한 흰쥐에서 갈근 추출물이 혈장 및 간장의 지질구성, 간장기능 및 항산화능에 미치는 영향. 한국자원식물학회지. 2007 ; 20(5) : 475-80.
  27. Bidlack WR, Tappel AL. Damage to microsomal membrane by lipid peroxidation. Lipids. 1973 ; 8(4) : 177-82. https://doi.org/10.1007/BF02544631