한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제42권3호
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  • Pages.238-242
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  • 2009
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
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양전하 및 음전하 필터를 이용한 해수 중 Norovirus Surrogate의 회수

Recovery of Norovirus Surrogate in Seawater using an Electropositive and Electronegative Filter

  • 이희정 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 오은경 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 유홍식 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 신순범 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 손명진 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 정진이 (국립수산과학원 양식연구부 식품안전연구과) ;
  • 김영목 (부경대학교 식품공학과) ;
  • 윤호동 (국립수산과학원 양식연구부 식품안전연구과)
  • Lee, Hee-Jung (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Oh, Eun-Gyoung (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Yu, Hong-Sik (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Shin, Soon-Bum (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Son, Myeong-Jin (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Jung, Jin-Yi (Food Safety Research Division, National Fisheries Research & Development Institute) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University) ;
  • Yoon, Ho-Dong (Food Safety Research Division, National Fisheries Research & Development Institute)
  • 발행 : 2009.06.30
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초록

Recently coastal seawater has been contaminated by enteric viruses such as the norovirus via untreated groundwater globally. Accordingly, the consumption of molluscan shellfish from seawater that has been contaminated with fecal material has become an important issues. The levels of enteric viruses in seawater are low and recovery and concentration of the virus from large volumes of water is difficult. We compared the effectiveness of two representative method of concentrating virus using negatively and positively charged filters. The mean retention of seeded FCV by HAMF and NCCF was 48% and 78%, respectively. Overall, the recovery of NCCF was 43.3$\pm$11% better than that of HAMF. However, the eluate obtained by using beef extract solution in the NCCF procedure caused an inhibitory effect on the RT-PCR; therefore, it was necessary to employ a PCR inhibitor removal procedure. The HAMF eluate contained no PCR inhibitors, but HAMF was not an effective method of concentrating the virus from large volumes of natural seawater due to clogging.

키워드

참고문헌

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피인용 문헌

  1. Negative Correlation between the Prevalence of Norovirus and High Bacterial Loads of Escherichia coli in Oysters Crassostrea gigas vol.18, pp.3, 2015, https://doi.org/10.5657/FAS.2015.0235
  2. Environmental Transmission of Noroviruses and Study of Fecal Microorgnisms as Viral Indicators in the Suyeong River in Busan, Korea vol.48, pp.3, 2009, https://doi.org/10.4167/jbv.2018.48.3.81