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http://dx.doi.org/10.5657/kfas.2009.42.3.238

Recovery of Norovirus Surrogate in Seawater using an Electropositive and Electronegative Filter  

Lee, Hee-Jung (Food Safety Research Division, National Fisheries Research & Development Institute)
Oh, Eun-Gyoung (Food Safety Research Division, National Fisheries Research & Development Institute)
Yu, Hong-Sik (Food Safety Research Division, National Fisheries Research & Development Institute)
Shin, Soon-Bum (Food Safety Research Division, National Fisheries Research & Development Institute)
Son, Myeong-Jin (Food Safety Research Division, National Fisheries Research & Development Institute)
Jung, Jin-Yi (Food Safety Research Division, National Fisheries Research & Development Institute)
Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
Yoon, Ho-Dong (Food Safety Research Division, National Fisheries Research & Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.42, no.3, 2009 , pp. 238-242 More about this Journal
Abstract
Recently coastal seawater has been contaminated by enteric viruses such as the norovirus via untreated groundwater globally. Accordingly, the consumption of molluscan shellfish from seawater that has been contaminated with fecal material has become an important issues. The levels of enteric viruses in seawater are low and recovery and concentration of the virus from large volumes of water is difficult. We compared the effectiveness of two representative method of concentrating virus using negatively and positively charged filters. The mean retention of seeded FCV by HAMF and NCCF was 48% and 78%, respectively. Overall, the recovery of NCCF was 43.3$\pm$11% better than that of HAMF. However, the eluate obtained by using beef extract solution in the NCCF procedure caused an inhibitory effect on the RT-PCR; therefore, it was necessary to employ a PCR inhibitor removal procedure. The HAMF eluate contained no PCR inhibitors, but HAMF was not an effective method of concentrating the virus from large volumes of natural seawater due to clogging.
Keywords
Norovirus; Enteric virus; PCR inhibitor; Negatively charged; Positively charged;
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