한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제42권5호
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  • Pages.341-347
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  • 2009
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

쌀겨와 밀기울의 토양 혐기발효 처리가 시설 재배지 토양의 미생물상에 미치는 영향

Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran

  • Kim, Hong-Lim (Namhae Sub-Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Weon, Hang-Yeon (Microbial Resources Team, National Agrobiodiversity Center, National Academy of Agricultural Science, RDA,) ;
  • Sohn, Bo-Kyun (Division of Applied Life and Environmental Sciences, Sunchon National University) ;
  • Choi, Young-Hah (Namhae Sub-Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwack, Young-Bum (Namhae Sub-Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 투고 : 2009.09.02
  • 심사 : 2009.10.12
  • 발행 : 2009.10.30
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초록

본 연구는 밀기울과 쌀겨를 이용한 토양 혐기발효 처리가 토양 미생물상 변화에 미치는 영향을 구명하고자 수행하였다. 희석 평판법을 이용하여 토양 미생물상을 분석한 결과 쌀겨를 처리한 토양은 사상균이 크게 증가한 반면, 효모의 생장은 확인할 수 없었다. 반면 밀기울을 처리한 토양은 사상균의 생장이 크게 억제되었으며, 효모는 높은 밀도를 보였다. 인지질 지방산을 이용하여 토양 미생물상을 분석한 결과, 처리가 진행되는 20일 까지는 밀기울과 쌀겨를 처리구 모두 그람 음성균과 양성균의 변동이 미미하였으나, 처리가 종료되어 비닐을 제거한 이후에는 크게 상승하는 경향을 보였다. 각 처리별 미생물 군집구조를 분석한 결과, 밀기울과 쌀겨 처리는 미생물상에 큰 변화를 보였으며, 각각의 군집구조는 크게 달랐다.

Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20 days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.

키워드

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