Journal of Microbiology and Biotechnology

  • 제19권5호
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  • Pages.455-461
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  • 2009
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Structural Characteristics of Immunostimulating Polysaccharides from Lentinus edodes

  • Lee, Hee-Hwan (Department of Bioengineering and Technology, Kangwon National University) ;
  • Lee, Jong-Seok (Department of Bioengineering and Technology, Kangwon National University) ;
  • Cho, Jae-Yeol (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Young-Eon (Korea Food Research Institute) ;
  • Hong, Eock-Kee (Department of Bioengineering and Technology, Kangwon National University)
  • 발행 : 2009.05.31
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초록

There is a significant amount of experimental evidence suggesting that polysaccharides from mushrooms enhance the host immune system by activating various mechanisms in immune cells, including macrophages. In this study, polysaccharides from Lentinus edodes were found to stimulate the functional activation of macrophages to secrete inflammatory mediators and cytokines and increase the phagocytotic uptake. The chemical properties of the stimulatory polysaccharides, CPFN-G-I, CPBN-G, and CPBA-G, were determined based on their monosaccharide composition, which mainly consisted of glucose and mannose. According to FT-IR and GC/MS, the structure of CPFN-G-I, purified from the fruiting body of L. edodes, was found to consist of a $\beta$-1,6-branched-$\beta$-1,4-glucan, whereas CPBN-G and CPBA-G, purified from the liquid culture broth, were found to be composed of a heteromannan. The configuration of the p-linkage and triple helical conformation of each polysaccharide were confirmed using a Fungi-Fluor kit and Congo red, respectively.

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