Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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Highly Fluorescing Solid DNA-Cationic Polyelectrolyte Complexes Prepared from a Natural DNA and a Poly(fluorenevinylene-alt-phenylene) Bearing Quaternary Ammonium Pendants

  • Yu, Young-Jun (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Kwon, Young-Wan (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Kim, Kyu-Nam (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Do, Eui-Doo (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Choi, Dong-Hoon (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Jin, Jung-Il (Department of Chemistry and Center for Advanced Materials Chemistry, Korea University) ;
  • Shin, Hee-Won (Department of Chemistry, Yonsei University) ;
  • Kim, Yong-Rok (Department of Chemistry, Yonsei University) ;
  • Kang, Ik-Joong (Department of Chemical and Bio Engineering, Kyungwon University) ;
  • Mikroyannidis, John A. (Chemical Technology Laboratory, Department of Chemistry, University of Patras)
  • Published : 2009.04.25
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Abstract

A fluorescing, copolymer(Q)-bearing, quaternary ammonium pendant was mixed with excess natural salmon sperm DNA with a molecular weight of $1.3{\times}10^6$(2,000 base pairs) to afford highly fluorescing, complex mixtures. The fluorescence life-time of the polymer Q was greatly increased when mixed with DNA: for the mixture of Q:DNA=1:750 the fast and slow decay lifetimes increased from ca. 10 to 100 ps and from 20 ps to ca. 1 ns, respectively. The enhanced fluorescence of the mixtures was ascribed to efficient compartmentalization and reduced conformational relaxation of the polymer Q by complexation with excess DNA.

Keywords

References

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