Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Cultivar Discrimination of Korean and Chinese Boxthorn (Lycium chinense Mill. and Lycium barbarum L.) using SSR Markers

SSR 마커를 이용한 한국산과 중국산 구기자의 품종 판별

  • Chung, Jong-Wook (Department of Plant resources, Kongju National University) ;
  • Lee, Gi-An (National Agrobiodiversity center, National Institute of Agricultural Biotechnology, RDA) ;
  • Lee, Sok-Su (Cheongyang Boxthorn Experiment station, CNARES) ;
  • Bang, Kyong-Hwan (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Chung-Berm (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Yong-Jin (Department of Plant resources, Kongju National University)
  • 정종욱 (공주대학교, 식물자원학과) ;
  • 이기안 (국립농업과학원, 농업유전자원센터) ;
  • 이석수 (충청남도농업기술원, 청양구기자시험장) ;
  • 방경환 (국립원예특작과학원, 인삼특작부) ;
  • 박충범 (국립원예특작과학원, 인삼특작부) ;
  • 박용진 (공주대학교, 식물자원학과)
  • Published : 2009.12.30
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Abstract

This study was undertaken to develop a technique of discrimination using SSR makers in boxthorn cultivars. Forty one boxthorn cultivars, which were collected from Korea and China, were evaluated by 10 SSR markers. Total of 61 alleles were detected, ranging from 3 to 13 with an average of 6.1 alleles per locus. The averages of gene diversity and PIC values were 0.482 and 0.428, with a range from 0.25 (GB-LCM-022 and GB-LCM-087) to 0.83 (GB-LCM-167) and from 0.24 (GB-LCM-022 and GB-LCM-087) to 0.81 (GB-LCM-167), respectively. Five markers out of 10 markers, GB-LCM-022, GB-LCM-075, GB-LCM-104, GB-LCM-167 and GB-LCM-217, were selected as key markers for discrimination in boxthorn cultivars. All of boxthorn cultivars were individually distinguished by the combination of five SSR markers.

Keywords

References

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