Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Genetic Diversity in Cultured and Wild Populations of the Ascidian Halocynthia roretzi Inferred from Mitochondrial DNA Analysis

  • Yoon, Moon-Geun (Faculty of Marine Bioscience and Technology, Kangnung- Worlju National University) ;
  • Lee, Joo-Kyung (Faculty of Marine Bioscience and Technology, Kangnung- Worlju National University) ;
  • Jin, Hyung-Joo (Faculty of Marine Bioscience and Technology, Kangnung- Worlju National University) ;
  • Jin, Deuk-Hee (Faculty of Marine Bioscience and Technology, Kangnung- Worlju National University)
  • Published : 2009.03.31
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Abstract

Nucleotide sequences of about 500 bp from the 5' end of mitochondrial (mt) DNA Cytochrome Oxidase I (COI) were analyzed to estimate the genetic variation between wild and cultured populations of the ascidian Halocynthia roretzi from two sites along the coast of Korea. A total of 25 haplotypes were defined by 21 variable nucleotide sites in the examined COI region. Genetic diversity (haplotype diversity and nucleotide divergence) of wild populations was higher than that of the cultured population. These data suggest that reduced genetic variation in the cultured population may have results from bottleneck effect caused by the use of a limited number of parental stock and pooling of gametes for fertilization. Pairwise population $F_{ST}$ estimates inferred that wild and cultured populations were genetically distinct. The combined results suggest that sequence polymorphism in the COI region would be preferable for estimating the genetic diversity of ascidian populations.

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