KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidant Activity of the Salt Marsh Plant Corydalis heterocarpa

염주괴불주머니 (Corydalis heterocarpa) 의 항산화 활성

  • Kim, You-Ah (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lee, Jung-Im (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lee, Jin-Hyeok (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Kong, Chang-Suk (Marine Bioprocess Research Center, Pukyong National University) ;
  • Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 김유아 (한국해양대학교 해양환경.생명과학부) ;
  • 이정임 (한국해양대학교 해양환경.생명과학부) ;
  • 이진혁 (한국해양대학교 해양환경.생명과학부) ;
  • 공창숙 (부경대학교 해양바이오프로세스연구단) ;
  • 남택정 (부경대학교 식품생명공학부) ;
  • 서영완 (한국해양대학교 해양환경.생명과학부)
  • Published : 2009.10.29
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Abstract

The antioxidant activities of two crude extracts ($CH_2Cl_2$ and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions) from Corydalis heterocarpa were determined by evaluating authentic $ONOO^-$ and $ONOO^-$ generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic $ONOO^-$ increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions, while those on $ONOO^-$ generated from SIN-1 increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at $10\;{\mu}M$. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.

Keywords

References

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