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Antioxidant Activity of the Salt Marsh Plant Corydalis heterocarpa  

Kim, You-Ah (Division of Marine Environment & Bioscience, Korea Maritime University)
Lee, Jung-Im (Division of Marine Environment & Bioscience, Korea Maritime University)
Lee, Jin-Hyeok (Division of Marine Environment & Bioscience, Korea Maritime University)
Kong, Chang-Suk (Marine Bioprocess Research Center, Pukyong National University)
Nam, Taek-Jeong (Faculty of Food Science and Biotechnology, Pukyong National University)
Seo, Young-Wan (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
KSBB Journal / v.24, no.5, 2009 , pp. 469-476 More about this Journal
Abstract
The antioxidant activities of two crude extracts ($CH_2Cl_2$ and MeOH) and their solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions) from Corydalis heterocarpa were determined by evaluating authentic $ONOO^-$ and $ONOO^-$ generated from SIN-1 (3-morpholinsydnonimine) in vitro as well as by measuring the degree of occurrence of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of solvent fractions on authentic $ONOO^-$ increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions, while those on $ONOO^-$ generated from SIN-1 increased in the order of n-BuOH > 85% aq. MeOH > $H_2O$ > n-hexane fractions. In addition, all solvent fractions effectively inhibited the intracellular ROS and NO levels. The n-BuOH fraction especially exhibited the strongest ROS scavenging effect. Further purification of n-BuOH fraction led to the isolation of cnidimoside A, which presented the potent ROS scavenging effect at $10\;{\mu}M$. From these results, extracts of C. heterocarpa and its component, cnidimoside A, were predicted to be potentially useful as ingredients for protecting against oxidation.
Keywords
Corydalis heterocarpa; cnidimoside A; nitric oxide; reactive oxygen species; peroxynitrite;
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