Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects

백서두개골 결손부에서 Escherichia coli 발현 시스템으로 생산된 rhBMP-2의 골형성 효과

  • Kwon, Suk-Hoon (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Lim, Hyun-Chang (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Choi, Kyung-Hee (Research Development Institute, Cowellmedi Co. LTD) ;
  • Kim, Min-Soo (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Lee, Ji-Hyun (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Jung, Ui-Won (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Yun, Jeong-Ho (Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital) ;
  • Kim, Chang-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration College of Dentistry, Yonsei University)
  • 권석훈 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 임현창 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 최경희 ((주) 코웰메디, 기술연구소) ;
  • 김민수 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 이지현 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 정의원 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 윤정호 (관동대학교 의과대학 명지병원 치과) ;
  • 김창성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소)
  • Published : 2009.03.31
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Abstract

Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.

Keywords

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