• 제목/요약/키워드: Ecoli expression system

검색결과 1건 처리시간 0.015초

백서두개골 결손부에서 Escherichia coli 발현 시스템으로 생산된 rhBMP-2의 골형성 효과 (Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects)

  • 권석훈;임현창;최경희;김민수;이지현;정의원;윤정호;김창성;최성호;조규성
    • Journal of Periodontal and Implant Science
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    • 제39권1호
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    • pp.77-86
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    • 2009
  • Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system. Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals. Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks. Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.