Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effects of Litsea japonica on the Induction of Apoptosis in HL-60/ADR

까마귀쪽나무(Litsea japonica)의 HL-60/ADR 세포 Apoptosis 유도효과

  • Kim, Elvira (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Boo, Hye-Jin (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Hyun, Jae-Hee (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kim, Sang-Cheol (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kang, Jung-Il (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kim, Min-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kang, Hee-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
  • 김엘비라 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 부혜진 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 현재희 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 김상철 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 강정일 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 김민경 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 유은숙 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소) ;
  • 강희경 (제주대학교 의학전문대학원 약리학교실, 의과학 연구소)
  • Published : 2009.03.31
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Abstract

The present study investigated the antiproliferative effect of Litsea japonica in HL-60/ADR, adriamycin resistant human promyelocytic leukemia cells. The 80% ethanol extract of L. japonica markedly inhibited the growth of HL-60/ADR cells. When HL-60/ADR cells were treated with the extract, several apoptosis events like as DNA fragmentation, chromatin condensation and the increase of the population of sub-G1 hypodiploid cells were observed. In the mechanism of apoptosis induction by L. japonica, we examined the changes of Bcl-2 and Bax protein expression levels, and activation of caspases. After the HL-60/ADR cells were treated with the extract, the Bcl-2 expression was decreased, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 and -3 were increased and the cleavage of poly (ADP-ribose) polymerase, a vital substrate of effector caspase, was observed. The results suggest that the inhibitory effect of L. japonica on the growth of the HL-60/ADR appears to arise from the induction of apoptosis via the down-regulation of Bcl-2 and the activation of caspases.

Keywords

References

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