Browse > Article

The Effects of Litsea japonica on the Induction of Apoptosis in HL-60/ADR  

Kim, Elvira (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Boo, Hye-Jin (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Hyun, Jae-Hee (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Kim, Sang-Cheol (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Kang, Jung-Il (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Kim, Min-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Kang, Hee-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.1, 2009 , pp. 65-69 More about this Journal
Abstract
The present study investigated the antiproliferative effect of Litsea japonica in HL-60/ADR, adriamycin resistant human promyelocytic leukemia cells. The 80% ethanol extract of L. japonica markedly inhibited the growth of HL-60/ADR cells. When HL-60/ADR cells were treated with the extract, several apoptosis events like as DNA fragmentation, chromatin condensation and the increase of the population of sub-G1 hypodiploid cells were observed. In the mechanism of apoptosis induction by L. japonica, we examined the changes of Bcl-2 and Bax protein expression levels, and activation of caspases. After the HL-60/ADR cells were treated with the extract, the Bcl-2 expression was decreased, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 and -3 were increased and the cleavage of poly (ADP-ribose) polymerase, a vital substrate of effector caspase, was observed. The results suggest that the inhibitory effect of L. japonica on the growth of the HL-60/ADR appears to arise from the induction of apoptosis via the down-regulation of Bcl-2 and the activation of caspases.
Keywords
Litsea japonica; HL-60/ADR; Apoptosis; Bcl-2; Caspases;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Min, B. S., Lee, S. Y., Kim, J. H., Kwon, O. K., Park, B. Y., An, R. B., Lee, J. K., Moon, H. I., Kim, T. J., Kim, Y. H., Joung, H. and Lee, H. K. (2003) Lactones from the leaves of the Litsea japonica and their anti-complement activity. J Nat Prod. 66(10): 1388-1390   DOI   ScienceOn
2 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-54   DOI   PUBMED   ScienceOn
3 Kroemer, G., Zamzami, N. and Susin, S. A. (1997) Mitochondrial control of apoptosis. Immunol Today. 18: 44-51   DOI   ScienceOn
4 Gottesman, M. M., Fojo, T. and Bates, S. E. (2002) Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer. 1: 8-58   DOI
5 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemisensitivity testing. Cancer Research. 47: 944-946
6 Tanaka, H., Nakamura, T. and Ichino, K. (1990) Butanolides from Litsea japonica. Phytochemistry. 29: 857-859   DOI   ScienceOn
7 Takeda, K., Sakurawi, K. and Ishii, H. (1972) Components of the Lauraceae family-l. New lactonic compounds from Litsea japonica. Tetrahedron. 28: 3757-3766   DOI   ScienceOn
8 Luo, Y. and Kessel, D. (1997) Initiation of apoptosis versus necrosis by photodynamic therapy with chloroaluminum phthalocyanine. Photochem Photobiol. 66: 479   DOI   ScienceOn
9 Kim, E., Boo, H. J., Hyun, J. H., Kim, S. C., Kang, J. I., Kim, M. K., Yoo, E. S. and Kang, H. K. (2009) The effect of Litsea japonica on the Apoptosis Induction of HL-60 leukemia cells. Yakhak Hoeji. 53(1): 6-11   ScienceOn
10 Lee, S. Y., Min, B. S., Kim, J. H., Lee, J., Kim, T. J., Kim, C. S., Kim, Y. H. and Lee, H. K. (2005) Flavonoids from the leaves of Litsea japonica and their anti-complement activity. Phytotherapy research. 19: 273-276   DOI   ScienceOn
11 Shen, D. W., Goldenberg, S., Pastan, I. and Gottesman M. M. (2000) Decreased accumulation of [14C] carboplatin in human cisplatin-resistant cells results from reduced energydependent uptake. J Cell Physiol. 183: 108-116   DOI   ScienceOn
12 Schuetz, E. G., Beck, W. T. and Schuetz, J. D. (1996) Modulators and substrates of P-glycoprotein and cytochrome P4503A coordinately up-regulate these proteins in human colon carcinoma cells. Mol Pharmacol. 49(2): 311-8   PUBMED   ScienceOn
13 Urasaki, Y., Laco, G. S., Pourquier, P., Takebayashi, Y., Kohlhagen, G., Gioffre, C., Zhang, H., Chatterjee, D., Pantazis, P. and Pommier, Y. (2001) Characterization of a novel topoisomerase I mutation from a camptothecin-resistant human prostate cancer cell line. Cancer Res. 61(5): 1964-9   PUBMED   ScienceOn
14 Goto, S., Kamada, K., Soh, Y., Ihara, Y. and Kondo, T. (2002) Significance of nuclear glutathione S-transferase pi in resistance to anti-cancer drugs. Jpn J Cancer Res. 93(9): 1047-56   DOI   PUBMED