Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
권호 표지
DOI QR코드

DOI QR Code

Detection of human and bovine haptoglobin by using quartz crystal microbalance sensor chip containing secondary antibody

이차항체를 포함하는 수정미소저울 센서 칩을 이용한 사람과 소의 헵토글로빈 측정

  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, secondary antibody-containing quartz crystal microbalance(QCM) sensor chip was prepared and utilized for the detection of human and bovine haptoglobin. Anti-goat immunoglobulin G antibody, which is a secondary antibody capable of capturing primary antibodies raised in goat, was immobilized through the reaction between hydrazide and aldehyde group prepared on the QCM surface and antibody respectively. The resulting sensor chip showed higher stability in the repeated surface regeneration with acidic dissociation solution as well as requiring lower amount of primary antibody when compared to the protein G sensor chip. The secondary antibody sensor chip was applied for the estimation of bovine and human haptoglobin.

Keywords

References

  1. H. C. Lu, H. M. Chen, Y. S. Lin, and J. W. Lin, 'A reusable and specific protein A-coated piezoelectric biosensor for flow injection immunoassay', Biotechnol. Prog., vol. 16, pp. 116-124, 2000 https://doi.org/10.1021/bp9901320
  2. W. Lee, B. K. Oh, Y. M. Bae, S. H. Paek, W. H. Lee, and J. W. Choi, 'Fabrication of self-assembled protein A monolayer and its application as an immunosensor', Biosens. Bioelectron., vol. 19, pp. 185-192, 2003 https://doi.org/10.1016/S0956-5663(03)00195-7
  3. J. W. Chung, J. M. Park, R. Bernhardt, and J. C. Pyun, 'Immunosensor with a controlled orientation of antibodies by using NeutrAvidin-protein. A complex at immunoaffinity layer', J. Biotechnol., vol. 126, pp. 325-333, 2006 https://doi.org/10.1016/j.jbiotec.2006.05.010
  4. 하인영, 최석정, 'Protein G를 포함하는 수정미소저 울 센서 칩과 정제되지 않은 항혈청을 이용한 헵토 글로빈과 트랜스페린의 면역분석', 센서학회지, 제 17권, 제5호, pp. 380-386, 2008 https://doi.org/10.5369/JSST.2008.17.5.380
  5. 김성일, 이현정, 박순혁, 방현수, 이진아, 최석정, '헵토글로빈 측정을 위한 수정미소저울 바이오센서 개발', 센서학회지, 제16권, 제2호, pp. 132-141, 2007 https://doi.org/10.5369/JSST.2007.16.2.132
  6. M. Kotaka, G. G. Chen, P. B. Lai, W. Y. Lau, P. K. Chan, T. W. Leung, and A. K. Li, 'Analysis of differentially expressed genes in hepatocellular carcinoma with hepatitis C virus by suppression subtractive hybridization', Oncol. Res., vol. 13, pp. 161-167, 2002
  7. Y. Kaneta, Y. Kagami, T. Tsunoda, R. Ohno, Y. Nakamura, and T. Katagiri, 'Genome-wide analysis of gene-expression profiles in chronic myeloid leukemia cells using a cDNA microarray', Int. J. Oncol., vol. 23, pp. 681-691, 2003
  8. B. Ye, D. W. Cramer, S. J. Skates, S. P. Gygi, V. Pratomo, L. Fu, N. K. Horick, L. J. Licklider, J. O. Schorge, R. S. Berkowitz, and S. C. Mok, 'Haptoglobin alpha subunit as potential serum biomarker in ovarian cancer: identification and characterization using proteomic profiling and mass spectrometry', Clin. Cancer. Res., vol. 9, pp. 2904-2911, 2003
  9. H. F. Juan, J. H. Chen, W. T. Hsu, S. C. Huang, S. T. Chen, J. Y.-C. Lin, Y. W. Chang, C. Y. Chiang, L. L. Wen, D. C. Chan, Y. C. Liu, and Y. J. Chen, 'Identification of tumor-associated plasma biomarkers using proteomic techniques: from mouse to human', Proteomics, vol. 4, pp. 2766-2775, 2004 https://doi.org/10.1002/pmic.200400785
  10. M. Nisnevitch and M. A. Firer, 'The solid phase in affinity chromatography: strategies for antibody attachment', J. Biochem. Biophys. Methods, vol. 49, pp. 467-480, 2001 https://doi.org/10.1016/S0165-022X(01)00214-7
  11. E. Briand, M. Salmain, J. M. Herry, H. Perrot, C. Compere and C. M. Pradier, 'Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?', Biosens. Bioelectron., vol. 22, pp. 440-448, 2006 https://doi.org/10.1016/j.bios.2006.05.018
  12. M. J. O’Brien II, S. R. J. Brueck, V. H. Perez-Luna, L. M. Tender, and G. P. Lopez, 'SPR biosensors: simultaneously removing thermal and bulk composition effects', Biosens. Bioelectron., vol. 14, pp. 145-154, 1999 https://doi.org/10.1016/S0956-5663(98)00121-3
  13. M. Åkerstedt, L. Bjorck, K. P. Waller, and Å. Sternesjo, 'Biosensor assay for determination of haptoglobin in bovine milk', J. Dairy Res., vol. 73, pp. 299-305, 2006 https://doi.org/10.1017/S0022029906001774