Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of a Bacillus subtilis Mannanase Gene in Corynebacterium lactofementum

Corynebacterium lactofermentum에서 Bacillus subtilis의 Mannanase 유전자 발현

  • Yoon, Ki-Hong (Department of Food Science & Biotechnology, Woosong University)
  • 윤기홍 (우송대학교 식품생물과학과)
  • Published : 2009.12.28
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Abstract

A Bacillus subtilis mannanase gene was subcloned into an Escherichia coli- Corynebacterium lactofermentum shuttle vector pHE83, and the resultant plasmid pHE83M was transferred into an endogenous plasmid-free strain of C. lactofermentum. Mannanase produced by the recombinant C. lactofermentum (pHE83M) was secreted extracellulary at the level of 86%, and the remaining activity of mannanase was detected in the cell-free extract. The maximum mannanase productivity of the recombinant strain reached 8.1 unit/mL in the culture filtrate of LB medium. According to the zymogram of mannanase on SDS-PAGE, it was found that the mannanase produced by the recombinant C. lactofermentum could be maintained stably with a migration identical to the mannanase produced by the parental strain, B. subtilis WL-3.

Keywords

References

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