Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

A Subgroup IB Isolate of Cucumber mosaic virus Isolated from Lagenaria leucantha var. gourda

표주박(Lagenaria leucantha var. gourda)에서 분리한 서브그룹 IB계통의 Cucumber mosaic virus

  • Oh, Sun-Mi (Department of Applied Biology, Kangwon National University) ;
  • Hong, Jin-Sung (Division of Environment & Life Sciences, Seoul Women's University) ;
  • Ryu, Ki-Hyun (Division of Environment & Life Sciences, Seoul Women's University) ;
  • Lee, Gung-Pyo (Division of Applied Plant Sciences, Chungang University) ;
  • Choi, Jang-Kyung (Department of Applied Biology, Kangwon National University)
  • 오선미 (강원대학교 응용생물학과) ;
  • 홍진성 (서울여자대학교 환경생명과학부) ;
  • 류기현 (서울여자대학교 환경생명과학부) ;
  • 이긍표 (중앙대학교 응용식물과학부) ;
  • 최장경 (강원대학교 응용생물학과)
  • Published : 2009.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

An isolate of Cucumber mosaic virus (CMV), called as Lag-CMV, was identified from Lagenaria leucantha var. gourda showing mosaic symptom, and its properties was compared to Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein gene. Lag-CMV was similar to As-CMV used as a control CMV by the induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves of N. tabacum. cv. Xanthi nc. In the cucumber and zucchini squash, Lag-CMV and As-CMV induced a mild mosaic symptoms than that of Fny-CMV. Size and shapes of local lesions on Chenophodium amaranticolor and Vigna unguiculata induced by Lag-CMV was similar those by Fny-CMV or As-CMV. In experiments of dsRNA profiles and RT-PCR analysis of coat protein gene, Lag-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using EcoRI, SalI, MspI, XhoI, and HindIII of the RTPCR products and nucleotide sequence analysis of the coat protein gene showed that Lag-CMV belong to a member of CMV subgroup IB of the same to As-CMV.

전형적인 모자이크 증상의 표주박(Lagenaria leucantha var. gourda)으로부터 CMV를 분리하고 Lag-CMV로 명명하였다. 표주박에서 분리한 Lag-CMV는 N. benthamiana에서 전신감염되어 전형적인 모자이크 증상을 나타내 대조로 공시한 CMV들과 유사하였다. 그러나 N. tabacum. cv. Xanthi nc에서 접종엽에 퇴록반점이 형성되는 점이 As-CMV와 유사하였으며, 접종엽에 무병징을 나타낸 Fny-CMV와 구분되었다. 또한 Fny-CMV에 감염된 오이와 쥬키니 호박은 매우 심한 모자이크 증상을 나타낸 반면, Lag-CMV와 As-CMV에 감염된 식물은 비교적 엷은 모자이크 병징이 발현되었다. 고추에서는 Fny-CMV와 As-CMV가 접종상엽에 모자이크 증상이 발현되으나, Lag-CMV는 무병징으로 감염되었다. Lag-CMV에 감염된 N. benthamiana로부터 추출한 dsRNA의 전기영동 패턴은 대조의 Fny-CMV나 As-CMV의 dsRNA와 종류 및 분자 크기에서 차이를 나타내지 않았다. Lag-CMV에 감염된 N. benthamiana로부터 추출한 total RNA를 외피단백질 유전자와 IR 및 3' 비번역영역 일부를 포함하는 약 950 bp의 cDNA합성을 위한 프라이머를 이용하여 RT-PCR을 실시한 결과 약 950 bp의 cDNA가 검출되었다. 이 cDNA를 제한효소 EcoRI, HindIII, MspI, SalI 및 XhoI으로 처리한 후 RFLP분석을 실시한 결과, Lag-CMV의 RFLP패턴은 As-CMV와 일치하였다. 한편 이 cDNA를 이용하여 염기서열을 분석한 결과, 염기서열의 유사도는 As-CMV와 99.1%, Fny-CMV와는 94.5%였으며, 외피단백질의 아미노산서열 유사도는 As-CMV는 100%, Fny-CMV와 97.3%를 나타냈다. 이와 같은 결과들로부터 표주박에서 분리한 Lag-CMV는 As-CMV와 같은 서브그룹 IB에 속하는 것으로 확인되었다.

Keywords

References

  1. Antignus, Y., Wang, Y., Perlsman, M., Lachman, O., Lavi, N. and Gal-On, A. 2001. Biological and molecular characterization of a new cucurbit-infecting Tobamovirus. Phtopathoiogy 91: 565-571 https://doi.org/10.1094/PHYTO.2001.91.6.565
  2. Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Korean J. Plant Pathol. 14: 7-12
  3. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83: 67-73 https://doi.org/10.1016/S0166-0934(99)00106-8
  4. Dodds, J. A. 1993. DsRNA in diagnosis. In : Diagnosis of Plant Virus Diseases, ed. by R.E.F. Matthews. pp. 273-295. CRC Press, Boca Raton, USA
  5. Edwards, M. C. and Gonsalves, D. 1983. Grouping of seven biologically defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73: 1117-1120 https://doi.org/10.1094/Phyto-73-1117
  6. Kim, D. H. and Lee, J. M. 2000. Seed treatment for Cucumber green mottle mosaic virus (CGMMV) in gourd (Lagenaria siceraria) seeds and its detection. J. Kor. Soc. Hort. Sci. 44: 1-6
  7. Kaper, J. M. and Waterworth, H. E. 1981. Cucumoviruses. In : Handbook of Plant Virus Infections, ed. by E. Kurstak, pp. 257-332. Elsevier/Holland Biolmedical, New York, USA
  8. Lee, G. P., Min, B.E., Kim, C. S., Choi, S. H., Ham, C. H., Kim, S. U. and Ryu, K. H. 2003. Plant virus cDNA chip hybridization for detection and differentiation of four cucubit-infecting tobamovirus. J. Virol. Methods 110: 19-24 https://doi.org/10.1016/S0166-0934(03)00082-X
  9. Lee, K. W., Lee, B. C., Park, H. C. and Lee, Y. S. 1990. Occurrence of cucumber green mottle mosaic virus disease of watermelon in Korea. Korean J. Plant Pathol. 6: 250-255
  10. Lisa, V., Boccardo, G., Dgostino, G. and Dellavlle, G. 1981. Characterization of a potyvirus causing zucchini yeiiow mosaic virus. Phytopathology 71: 667-672 https://doi.org/10.1094/Phyto-71-667
  11. Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69: 854-858 https://doi.org/10.1094/Phyto-69-854
  12. Owen, J. and Palukaitis, P. 1988. Characterization of cucumber mosaic virus. I. Molecular heterogenecity mapping of RNA3 in eight CMV strains. Virology 166: 496-502
  13. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus. Res. 41: 281-348 https://doi.org/10.1016/S0065-3527(08)60039-1
  14. Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, M. R. 1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. J. Gen. Virol. 73: 2009-2103
  15. Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangement in the 5' nontranslated region and phylogenetic analysis of cucumber mosaic virus RNA3 indicated radial evolution of three subgroups. J. Virol. 73: 6752-6758
  16. Ryu, K. H., Min, B. E., Choi, G. S., Choi, S. H., Kwon, S. B., Noh, G. M., Yoon, J. Y., Choi, Y. M., Jang, S. H., Lee, G. P., Cho, K. H. and Park, W. M. 2000. Zucchini green mottle mosaic virus is a new Tabamovirus; Comparison of its coat protein gene with that of Kyuri green mottle mosaic virus. Arch. Virol. 145: 2325-2333 https://doi.org/10.1007/s007050070023
  17. Singh, Z., Jones, R. A. C. and Jones, M. G. K. 1995. Identification of Cucumber mosaic virus subgroup I isolates from banana plants affected by infectious chlorosis disease using RT-PCR. Plant Dis. 79: 713-716
  18. Tomlinson, J. A. 1987. Epidemiology and control of virus diseases of vegetables. Ann. Appl. Biol. 110: 661-681 https://doi.org/10.1111/j.1744-7348.1987.tb04187.x
  19. Wang, W. Q., Natsuaki, T., Okuda, S. and Teranaka, M. 1988. Comparison of cucumber mosaic virus isolate by duoblestrand RNA analysis. Ann Phytopath. Soc. Japan 54: 536-539 https://doi.org/10.3186/jjphytopath.54.536