Mycobiology

The Korean Society of Mycology (한국균학회)
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Ergosterol and Water Changes in Tricholoma matsutake Soil Colony during the Mushroom Fruiting Season

  • Published : 2009.03.31
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Abstract

The purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in Tricholoma matsutake soil colonies during the mushroom fruiting season. The active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular T. matsutake colony at Ssanggok valley in the Sogri Mt. National Park in Korea during 2003 to 2005. The four points were the ahead of the colony, the front edge of the colony and 20 cm and 40 cm back from the front edge of the colony. Ergosterol content was 0.0 to 0.7 ${\mu}g$ per gram dried soil at the ahead, 2.5 to 4.8 ${\mu}g$ at the front edge, 0.5 to 1.8 ${\mu}g$ at the 20 cm back and 0.3 to 0.8 ${\mu}g$ at the 40 cm back. The ergosterol content was very high at the front edge where the T. matsutake hyphae were most active. However, ergosterol content did not significantly change during the fruiting season, September to October. Soil water contents were lower at the front edge and 20 cm back from the front edge of the colony than at the ahead and 40 cm back during the fruiting season. Soil water content ranged from 12 to 19% at the ahead, 10 to 11% at the edge, 9 to 11% at the 20 cm back and 11 to 15% at the 40 cm back. Our results suggest that the active front edge of the T. matsutake soil colony could be managed in terms of water relation and T. matsutake ectomycorrhizal root development.

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References

  1. Antibus, R. K. and Sinsabaugh, R. L. 1993. The extraction and quantification of ergosterol from ectomycorrhizal fungi and roots. Mycorrhiza 3:137-144 https://doi.org/10.1007/BF00208921
  2. Bak, W. C. and 21 people. 2006. Development of cultivation methods for improving productivity of pine mushroom. Korean Forest Research Institute, Ministry of Agriculture and Forestry. pp. 8-34. Seoul, Korea
  3. Bentz, B. J. and Six, D. L. 2006. Ergosterol content of fungi associated with Dendronoctonus pondersosae and Dendronoctonus fufipennis (Coleoptera: Curculionidae, Scolytinae). Ann. Entomol. Soc. Am. 99:189-194 https://doi.org/10.1603/0013-8746(2006)099[0189:ECOFAW]2.0.CO;2
  4. Davis, M. W. and LaMar, R. T. 1992. Evaluation of methods to extract ergosterol for quantification of soil fungal biomass. Soil Biol. Biochem. 24:189-198 https://doi.org/10.1016/0038-0717(92)90218-M
  5. Ekblad, A., Wallander, H. and Nasholm, T. 1998. Chitin and ergosterol combined to measure total and living fungal biomass in ectomycorrhizas. New Phytol. 138:143-149 https://doi.org/10.1046/j.1469-8137.1998.00891.x
  6. Grant, W. D. and West, A. W. 1986. Measurement of ergosterol, diaminopimelic acid and glucosamine in soil: evaluation as indicators of microbial biomass. J. Microbial. Methods 6:47-53 https://doi.org/10.1016/0167-7012(86)90031-X
  7. Guerin-Laguette, A., Matsushita, N. Lapeyrie, F., Shindo, K. and Suzuki, K. 2005. Successful inoculation of mature pine with Tricholoma matsutake. Mycorrhiza 15:301-305 https://doi.org/10.1007/s00572-005-0355-4
  8. Hart, M. R. and Brookes, P. C. 1996. Effects of two ergosterolinhibiting fungicides on soil ergosterol and microbial biomass. Soil. Biol. Biochem. 28:885-892 https://doi.org/10.1016/0038-0717(96)00071-5
  9. Hosford, D., Pilz, D., Molina, R. and Amaranthus, M. 1997. Ecology and management of the commercially harvested American matsutake. Gen. Tech. Rep. PNW-GTR-412, pp. 1-68. Pacific Northwest Research Station, USDA Forest Service. Portland, OR.
  10. Hur, T. C., Park, H., Ka, K. H. and Joo, S. H. 2004. Dynamic changes of soil physicochemical properties in the fairy-rings of Tricholoma matsuatke. J. Kor. For. Soc. 93:26-34
  11. Ka, K. H., Hur, T. C., Park, H., Kim, H. S., Bak, W. C. and Yoon, K. H. 2006. Production and transplanting of ectomycorrhizal pine seedlings using the old fairy ring of Tricholoma matsutake. J. Kor. For. Soc. 95:636-642
  12. Kim, J. S., Cho, J. M., Kim, S. B., Kim, H. J., Jung, T. K., Koo, C. D. and Park, H. 1999. Pine mushroom, Its Sustainable production strategy. Sin Nonmin Lecture Series. No 38. Nonmin sinmunsa, pp. 33-41. Seoul, Korea
  13. Koo, C. D. and Bilek, E. M. 1998. Financial analysis of vegetation control for sustainable production of Songyi (Tricholoma matsutake) in Korea. J. Kor. For. Soc. 87:519-527
  14. Koo, C. D., Kim, J. S., Lee, S. H., Park, J. I. and Ahn, K. T. 2003. Spatio-temporal soil water changes in fairy ring colony of Tricholoma matsutake. J. Kor. For. Soc. 92:632-641
  15. Koo, C. D. 2005. Morphological characteristics of Tricholoma matsuake ectomycorrhiza. J. Kor. For. Soc. 94:16-20
  16. Koo, C. D., Ka, K. H., Park, W. C., Park, H., Ryu, S. R., Park, Y. W. and Kim, T. H. 2007. Changes of leaf area index, physiological activities and soil water in Tricholoma matsutake producing pine forest ecosystem. J. Kor. For. Soc. 96:438-447
  17. Kyoto Forest Research Institute. 1982. Matsutake. Japanese Kyoto Forest Research Institute Research Series No 6. pp. 1-26. Kyoto, Japan
  18. Lee, W. Y., Ahn, J. K., Ka, K. H. and Kwon, Y. J. 2003. Comparison of growth characteristics of Tricholoma matsutake mycelium among the types of air bubble bioreactor. Kor. J. Mycol. 31:89-93 https://doi.org/10.4489/KJM.2003.31.2.089
  19. Matchan, S. E., Jordan, B. R. and Wood, D. A. 1985. Estimation of fungal biomass in a solid substrate by three independent methods. Appl. Microb. Biotech. 21:108-112
  20. Newell, S. Y. 1992. Estimating fungal biomass and productivity in decomposing litter. In Yhe fungal community: Its organization and role in the ecosystem, pp. 521-561. Eds. G. C. Carroll and D. T. Wicklow. Dekker, New York
  21. Nylund, J. E. and Wallander, H. 1992. Ergosterol analysis as a means of quantifying mycorrhizal biomass. In Methods in Microbiology 24, pp. 77-88. Eds. J. R. Norris, D. J. Read, and A.K. Varma. Academic Press, Tokyo
  22. Ogawa, M. 1975. Mcrobial ecology of mycorrhizal fungus, Tricholoma matsutake Ito et Imai (Sing.) in pine forest. I. Fungal colony (shiro) of Tricholoma matsutake. Bull. Gov. For. Exp. Sta. 272:79-121
  23. Ogawa, M. 1991. Biology of Matsutake. 2nd edition. pp. 32-228. Tsukiji Shokan Co. Ltd, Tokyo
  24. Park, H., Ka, K. H., Hur, T. C. Hong, Y. P., Bak, W. C. and Yeo, U. H. 2004. Occurrence of fruiting body of Tricholoma matsutake at a Pinus rigida stand in Korea. J. Kor. For. Soc. 93:401-408
  25. Park, H., Kim, K. S. and Koo, C. D. 1995. Effects of climatic condition in September on pine mushroom (Tricholoma matsutake) yield and a method for overcoming the limiting factors in Korea. J. Kor. For. Soc. 84:479-488
  26. Parsi, Z. and Gorecki, T. 2006. Determination of ergosterol as an indicator of fungal biomass in various samples using non-discriminating flash pyrolysis. J. Chromatography A. 1130:145-150 https://doi.org/10.1016/j.chroma.2006.07.045
  27. Satomura, T., Hashimoto, Y., Kinoshita, A. and Horikoshi, T. 2006. Methods to study the role of ectomycorrhizal fungi in forest carbon cycling 2: Ergosterol analysis method to quantify the fungal content in ectomycorrhizal fine roots. Root Res. 15:149-154 https://doi.org/10.3117/rootres.15.149
  28. Suzuki, K. 2005. Ectomycorrhizal ecophysiology and the puzzle of Tricholoma matsutake. J. Jpn. For. Soc. 87:90-102 https://doi.org/10.4005/jjfs.87.90
  29. Weete, J. D. 1989. Structure and function of sterols in fungi. Adv. Lipid Res. 23:115-167
  30. West, A. W., Grant, W. D. and Sparling, G. P. 1987. Use of ergosterol, diaminopimelic acid and glucosamine contents of soil to monitor changes in microbial populations. Soil Biol. Biochem. 19:607-612 https://doi.org/10.1016/0038-0717(87)90106-4
  31. Yamada, A., Kanekawa, S. and Ohmasa, M. 1999. Ectomycorrhiza formation of Tricholoma matsutake isolates on seedlings of Pinus densiflora in vitro. Mycoscience 40:455-463 https://doi.org/10.1007/BF02461022