Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of a Sapstaining Fungus, Ophiostoma floccosum, Isolated from the Sapwood of Pinus thunbergii in Korea

  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

An Ophiostoma fungus was isolated from a stump of Pinus thunbergii in a forest on the West coast of Korea. Microscopic analysis using a light microscope, a stereo microscope, and a scanning electron microscope revealed that it had morphological features of Pesotum and Sporothix synanarmorphs. Based on the $\beta$-tubulin gene sequence analysis, the fungus was identified as the anamorph of Ophiostoma floccosum. Mycological properties of the species including its growth properties on different culture media were described.

Keywords

References

  1. Abraham, L. D., Roth, A., Saddler, J. N. and Breuil, C. 1993. Growth, nutrition, and proteolytic activity of the sap-staining fungus Ophiostoma piceae. Can. J. Bot. 71:1224-1230. https://doi.org/10.1139/b93-144
  2. Chung, W. H., Kim, J. J., Yamaoka, Y., Uzunovic, A., Masuya, H. and Breuil, C. 2006. Ophiostoma breviusculum sp. nov.(Ophiostomatales, Ascomycota) is a new species in the Ophiostoma piceae complex associated with bark beetles infesting larch in Japan. Mycologia 98:801-814. https://doi.org/10.3852/mycologia.98.5.801
  3. Harrington, T. C., McNew, D., Steimel, J., Hofstra, D. and Farrell, R. 2001. Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi. Mycologia 93:111-136. https://doi.org/10.2307/3761610
  4. Hunt, J. 1956. Taxonomy of the genus Ceratocystis. Lloydia 19:1-59
  5. Kim, S. H., Uzunovic, A. and Brueil, C. 1999. Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR. Appl. Environ. Microbiol. 65:287-290
  6. Kim, S. H., Kim, J. J., Lee, S. W. and Brueil, C. 2003. Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi. FEMS Microbiol. Lett. 222:187-192 https://doi.org/10.1016/S0378-1097(03)00304-5
  7. O'Donnell, K. and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS 2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol. Phylogenet. Evol. 7:103-116 https://doi.org/10.1006/mpev.1996.0376
  8. Seifert, K. A. and Okada, G. 1993. Graphium anamorphs of Ophiostoma species and similar anamorphs of other ascomycetes. In: Wingfield, M. J., Seifert, K. A. and Webber, J. F., eds. Ceratocystis and Ophiostoma: Taxanomy, ecology and Pathogenecity. St. Poul, Minnesota: American Phytopathological Society Press. pp. 27-41
  9. Schirp, A., Farrell, R. L. and Kreber, B. 2003. Effects of New Zealand sapstaining fungi on structural integrity of unseasoned radiate pine. Holz Roh-Werkst. 61:369-376 https://doi.org/10.1007/s00107-003-0402-9
  10. Swofford, D. L. 2003. PAUP*: Phylogenetic analysis using parsimony(* and other methods), version 4.0b10. Sinauer Associates, Sunderland, Massachusetts
  11. Uzunovic, A., Seifert, K., Kim, S. H. and Breuil, C. 2000. Ophiostoma setosum, a common sapwood staining fungus from western North America, a new species of Ophiostoma piceae complex. Mycol. Res. 104:387-394
  12. Wingfield, M. J., Seifert, K. A. and Webber, J. F. 1993. Ceratocystis and Ophiostoma Taxonomy, Ecology and Pathogenicity. pp. 27-60. American Phytopathological Society Press, Minnesota, USA