Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Quantitative Differences in mRNA Expression of Toll-like Receptor (TLR)-2, -4, and -9 in Normal Equine Eyes and Eyes with Equine Recurrent Uveitis

말의 정상안과 재발성 포도막염이 있는 안구에서의 Toll-like Receptor-2, -4, -9 발현 비교

  • Yi, Na-Young (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University) ;
  • Salmon, Jacklyn (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University) ;
  • Gilger, Brian (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University)
  • 이나영 (노스캐롤라이나 수의과대학, 비교 안과 연구 실험실) ;
  • ;
  • Published : 2009.12.31
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Abstract

The purporse of this study was to evaluate the quantitative differences in mRNA expression of TLR-2, -4, and -9 in normal equine eyes and eyes with equine recurrent uveitis (ERU). Normal equine eyes (n = 6) and eyes with naturally-occurring ERU (n = 6) were collected. Real time PCR assay was performed to compare mRNA expression of TLR-2, -4, and -9 between normal and ERU eyes. A significant up-regulation of TLR-2 and -9 mRNA in the ciliary body and TLR-2 mRNA in the iris was found in eyes with ERU compared to the mRNA levels in these same tissues of normal equine eyes. There were no remarkable differences observed in TLR-4 mRNA expression between normal eyes and eyes with ERU. The current data suggest the potential involvement of TLR-2 and -9 in the pathogenesis of ERU. However, further study is required to determine the role of TLRs in ERU.

본 연구는 말의 정상안과 재발성 포도막염이 있는 안구에서의 TLR-2, -4, -9 mRNA 발현의 정량적 차이를 비교하기 위해 수행되었다. 정상 및 재발성 포도막염이 있는 말 각 6두 에서 안구를 적출하여 모양체, 홍채, 망막 및 맥락막을 수집하였다. Real-time PCR assay 통해 정상안과 재발성 포도막염이 있는 안구에서의 TLR-2, -4, -9의 mRNA 발현 차이를 정량적으로 비교하였다. 말의 재발성 포도막염 시에는 모양체, 홍채에서 정상인 경우에 비해 4-12 배의 TLR-2 와 TLR-9 mRNA 발현증가를 보였으며, 맥락막 및 망막에서는 2-6 배의 TLR-2, -4, -9 mRNA 발현 증가를 보였다. 본 연구 결과는 Toll-like receptor 2, -4, -9이 말의 재발성 포도막염의 병리기전에 영향을 미치고 있음을 시사한다. 하지만 재발성 포도막염 시의 Toll-like receptor 2, -4, -9의 구체적 역할을 밝히기 위해서는 다양한 후속 연구가 요구된다.

Keywords

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