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Quantitative Differences in mRNA Expression of Toll-like Receptor (TLR)-2, -4, and -9 in Normal Equine Eyes and Eyes with Equine Recurrent Uveitis  

Yi, Na-Young (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University)
Salmon, Jacklyn (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University)
Gilger, Brian (Comparative Ophthalmology Research Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University)
Publication Information
Journal of Veterinary Clinics / v.26, no.6, 2009 , pp. 520-523 More about this Journal
Abstract
The purporse of this study was to evaluate the quantitative differences in mRNA expression of TLR-2, -4, and -9 in normal equine eyes and eyes with equine recurrent uveitis (ERU). Normal equine eyes (n = 6) and eyes with naturally-occurring ERU (n = 6) were collected. Real time PCR assay was performed to compare mRNA expression of TLR-2, -4, and -9 between normal and ERU eyes. A significant up-regulation of TLR-2 and -9 mRNA in the ciliary body and TLR-2 mRNA in the iris was found in eyes with ERU compared to the mRNA levels in these same tissues of normal equine eyes. There were no remarkable differences observed in TLR-4 mRNA expression between normal eyes and eyes with ERU. The current data suggest the potential involvement of TLR-2 and -9 in the pathogenesis of ERU. However, further study is required to determine the role of TLRs in ERU.
Keywords
Toll-like receptors; mRNA; recurrent uveitis; immune-mediated; horse;
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