Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of GuBoEum Inhibiting NO, TNF-$\alpha$, IL-6 and IL-12 Production by Blocking MAP Kinase Activation in LPS-induced Murine Macrophages

LPS로 유도한 대식세포에서 MAP kinase의 억제에 의한 구보음(九寶飮)의 NO, TNF-$\alpha$, IL-6, IL-12 생성 억제 효과

  • Lee, Byung-Soon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Jo-Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Si-Hyeong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 이병순 (원광대학교 한의과대학 폐계내과학교실) ;
  • 신조영 (원광대학교 한의과대학 폐계내과학교실) ;
  • 이시형 (원광대학교 한의과대학 폐계내과학교실)
  • Published : 2009.02.25
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Abstract

The purpose of this study was to investigate the anti-inflammatory effects of extract from GuBoEum(GBE) on the peritoneal macrophage. To evaluate anti-inflammatory effects of GBE. I measured cytokines (interleukin-6; IL-6, interleukin-12; IL-12, tumor necrosis factor-$\alpha$; TNF-$\alpha$) and nitric oxide (NO) production in lipopolysacchride (LPS)-induced macrophages. Furthermore, I examined molecular mechanism using western blot and also LPS-induced endotoxin shock. Extract from GBE does not have any cytotoxic effect in the peritoneal macrophages. Extract from GBE reduced LPS-induced IL-6, TNF-$\alpha$, IL-12 and NO production in peritoneal macrophages. GBE inhibited the activation of extracelluar signal-regulated kinase (ERK), C-Jun $NH_2$-terminal kinase (JNK) but not of p38, degradation of $I{\kappa}B-{\alpha}$ in the LPS-stimulated peritoneal macrophages. GBE inhibited the production of TNF-$\alpha$, IL-6 and IL-12 in serum after LPS injection. These results suggest that GBE may inhibit the production of TNF-$\alpha$, IL-6, and IL-12 through inhibition of ERK and JNK activation, and that GBE may be beneficial oriental medicine for inflammatory diseases.

Keywords

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