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Effects of GuBoEum Inhibiting NO, TNF-$\alpha$, IL-6 and IL-12 Production by Blocking MAP Kinase Activation in LPS-induced Murine Macrophages  

Lee, Byung-Soon (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Shin, Jo-Young (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Lee, Si-Hyeong (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.1, 2009 , pp. 104-112 More about this Journal
Abstract
The purpose of this study was to investigate the anti-inflammatory effects of extract from GuBoEum(GBE) on the peritoneal macrophage. To evaluate anti-inflammatory effects of GBE. I measured cytokines (interleukin-6; IL-6, interleukin-12; IL-12, tumor necrosis factor-$\alpha$; TNF-$\alpha$) and nitric oxide (NO) production in lipopolysacchride (LPS)-induced macrophages. Furthermore, I examined molecular mechanism using western blot and also LPS-induced endotoxin shock. Extract from GBE does not have any cytotoxic effect in the peritoneal macrophages. Extract from GBE reduced LPS-induced IL-6, TNF-$\alpha$, IL-12 and NO production in peritoneal macrophages. GBE inhibited the activation of extracelluar signal-regulated kinase (ERK), C-Jun $NH_2$-terminal kinase (JNK) but not of p38, degradation of $I{\kappa}B-{\alpha}$ in the LPS-stimulated peritoneal macrophages. GBE inhibited the production of TNF-$\alpha$, IL-6 and IL-12 in serum after LPS injection. These results suggest that GBE may inhibit the production of TNF-$\alpha$, IL-6, and IL-12 through inhibition of ERK and JNK activation, and that GBE may be beneficial oriental medicine for inflammatory diseases.
Keywords
GuBoEum(GBE); oriental medicine;
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1 東醫寶鑑國譯委員會. 對譯東醫寶監. 서울, 법인문화사, p 1232, 1243, 1999
2 申天浩 編譯. 病症 診治. 서울, 成輔社, p 151, 1990
3 新谷太. Pathophysiology로 이해하는 내과학 (감염학). 서울, 정담, p 9, 2002
4 Horwood, N.J., Page, T.H., McDaid, J.P., Palmer, C.D., Campbell, J., Mahon, T., Brennan, F.M., Webster, D., Foxwell, B.M. Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production. J Immunol. 176(6):3635-3641, 2006   DOI
5 Chen, C.H., Sheu, M.T., Chen, T.F., Wang, Y.C., Hou, W.C., Liu, D.Z., Chung, T.C., Liang, Y.C. Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways. Biochem Pharmacol. 72(8):1001-1009, 2006   DOI   ScienceOn
6 洪素蘭, 陳永輝, 裴曉華. 實用中醫呼吸病學. 北京, 中國中醫藥出版社, p 65, 67, 68, 1995
7 安德均. 韓國本草圖鑑. 서울, 교학사, p 40, 45, 46, 434, 463, 602, 739, 1999
8 金頀哲. 韓藥藥理學. 서울, 집문당, p 63, 68, 82, 252, 266, 278, 364, 434, 490, 2001
9 Lee, B.G., Kim, S.H., Zee, O.P., Lee, K.R., Lee, H.Y., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur J Pharmacol. 406: 301-309, 2000   DOI   ScienceOn
10 Nathan, C. Inducible nitric oxide synthase: what difference does it make? J Clin Invest. 100(10):2417-2423, 1997   DOI   ScienceOn
11 Durham, S.R., Ying, S., Varney, V.A., Jacobson, M.R., Sudderick, R.M., Mackay, I.S., Kay, A.B., Hamid, Q.A. Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of cells expressing messenger RNA for interferon-gamma. J Allergy Clin Immunol, 97(6):1356-1365, 1996   DOI   ScienceOn
12 李尙仁외. 韓藥臨床應用. 서울, 성보사, p 36, 39, 47, 52, 205, 225, 229, 323, 389, 475, 481, 1998
13 Kostova, I., Momekov, G., Tzanova, T., Karaivanova, M. Synthesis, Characterization, and Cytotoxic Activity of New Lanthanum(III) Complexes of Bis-Coumarins. Bioinorg Chem Appl. 25651: 1-9, 2006
14 Nathan, C., Xie, Q.W. Nitric oxide synthases: roles, tolls and controls. Cell. 78: 915-918, 1994   DOI   ScienceOn
15 과학기술부. 염증조절기작에 의거한 항염증 물질 검색시스템 및 항염증제 개발, 2002
16 Kawai, T., Adachi, O., Ogawa, T., Takeda, K., Akira, S. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity. 11(1):115-122, 1999   DOI   ScienceOn
17 Beutler, B., Cerami, A. The biology of cachectin/ TNF-$\alpha$ primary mediator of the host response. Annu Rev. Immunol. 7: 625-655, 1989   DOI   PUBMED   ScienceOn
18 劉河間. 劉河間傷寒三六書. 서울, 성보사, p 77, 1976
19 Hirano, T., Yasukawa, K., Harada, H., Taga, T., Watanabe, Y., Matsuda, T., Kashiwamura, S., Nakajima, K., Koyama, K., Iwamatsu, A. et al. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature, 324(6092):73-76, 1986   DOI   ScienceOn
20 Gajewski, T.F., Fitch, F.W. Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones. J Immunol. 140(12):4245-4252, 1988
21 Nishimoto, N., Kishimoto, T., Yoshizaki, K. Anti-interleukin 6 antibody treatment in rheumatic disease. Ann Rheum Dis. 59: i21-27, 2000   DOI   PUBMED
22 Huh, J.E., Yim, J.H., Lee, H.K., Moon, E.Y., Rhee, D.K., Pyo, S. Prodigiosin isolated from Hahella chejuensis suppresses lipopolysaccharide-induced NO production by inhibiting p38 MAPK, JNK and NF-kappaB activation in murine peritoneal macrophages. Int Immunopharmacol. 7(13):1825-1833, 2007   DOI   PUBMED   ScienceOn
23 Stankiewicz, W., Dabrowski, M.P., Chcialowski, A., Plusa, T. Cellular and cytokine immunoregulation in patients with chronic obstructive pulmonary disease and bronchial asthma. Mediators Inflamm. 11(5):307-312, 2002   DOI   ScienceOn
24 Stephen, T.. Holgate. The epidemic of allergy and asthma. Nature. 402: B2-4, 1999   DOI   ScienceOn
25 吳克潛. 古今醫方集成 卷2. 서울, 성보사, p 39, 1980
26 Seo, W.G., Pae, H.O., Oh, G.S., Kim, N.Y., Kwon, T.O., Shin, M.K., Chai, K.Y., Chung, H.T. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophage. J Ethnophamacol. 76: 119-123, 2001   DOI   ScienceOn
27 이경희, 김홍렬, 정희재, 이형구. LPS로 유발된 대식세포의 염증반응에 대한 淸上補下湯의 효과. 대한한방내과학회지 29(1):12-24, 2008   과학기술학회마을
28 Akira, S., Takeda, K., Kaisho, T. Toll-like receptors : critical proteins linking innate and acquired immunity. Nat Immunol. 2(8):675-680, 2001   DOI   ScienceOn
29 Vilcek, J., Lee, T.H. Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions, J Biol Chem, 266(12):7313-7316, 1991   PUBMED
30 Bhattacharyya, A., Pathak, S., Datta, S., Chattopadhyay, S., Basu, J., Kundu, M. Mitogen-activated protein kinases and nuclear factor-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 368: 121-129, 2002   DOI   ScienceOn
31 歐陽忠興, 柯新橋. 中醫呼吸病學. 北京, 中國醫藥科技出版社, pp 53-55, 1994
32 전국한의과 대학 폐계내과학 교실 편저. 동의폐계내과학. 서울, 한 문화사, p 147, 148, 163, 2002
33 이승우, 한상환. 九寶飮이 Sensitized Rat의 氣管支平滑筋에 미치는 影響. 대한한방내과학회지 14(2):1-19, 1993   과학기술학회마을
34 Lee, S.J., Lim, K.T. Glycoprotein isolated from Ulmus davidiana Nakai regulates expression of iNOS and COX-2 in vivo and in vitro. Food Chem Toxicol. 45(6):990-1000, 2007   DOI   ScienceOn
35 Moncada, S., Palmer, R.M., Higgs, E.A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev. 43: 109-142, 1991   PUBMED
36 Brenman, J.E., Chao, D.S., Gee, S.H., McGee, A.W., Craven, S.E., Santillano, D.R., Wu, Z., Huang, F., Xia, H., Peters, M.F., Froehner, S.C. and Bredt, D.S. Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and a 1-syntrophin mediated by PDZ domains. Cell. 84: 757-767, 1996   DOI   PUBMED   ScienceOn
37 이정재. a-MSH 유도성 멜라닌 합성에 있어서 감초 추출물의역할과 작용기전. 전북대학교 대학원, 2001
38 김정제 편저. 진료요감. 서울, 동양의학연구소, 상권 p 589, 하권 p 417, 1983
39 Eigler, A., Sinha, B., Hartmann, G., Endres, S. Taming TNF: strategies to restrain this proinflammatory cytokine, Immunol. Today. 18(10):487-492, 1997   DOI   ScienceOn
40 김규동. 동의내과학. 서울, 여강출판사, p 129, 1992
41 蘇軾, 沈括. 蘇沈良方(欽定四庫全書 卷6). 서울, 대성문화사, p 251, 1995
42 이병삼, 김홍렬, 김진주, 정승기, 이형구, 정희재. 解熱湯이 LPS로 자극된 대식세포에 있어 염증관련 Cytokine 발현억제에 미치는 효과. 대한한방내과학회지 29(2):334-347, 2008   과학기술학회마을
43 辛民敎. 臨床本草學. 서울, 영림출판사, p 175, 254, 261, 380, 382, 519, 528, 564, 581, 599, 1989
44 Mogensen, T.H., Paludan, S.R. Molecular pathways in virus-induced cytokine production, Microbiol. Mol. Biol. Rev. 65(1):131-150, 2001   DOI   ScienceOn