Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Synergistic Effect of Dexamethasone and Prolactin on VEGF Expression in Bovine Mammary Epithelial Cells via p44/p42 MAP Kinase

  • Nakajima, Kei-Ichi (National Agricultural Research Center for Hokkaido Region National Agriculture and Food Research Organization) ;
  • Nakamura, Masato (National Agricultural Research Center for Hokkaido Region National Agriculture and Food Research Organization) ;
  • Ishisaki, Akira (Department of Biochemistry, Iwate Medical University School of Dentistry) ;
  • Kozakai, Takaharu (National Agricultural Research Center for Hokkaido Region National Agriculture and Food Research Organization)
  • Received : 2008.09.17
  • Accepted : 2009.02.07
  • Published : 2009.06.01
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Abstract

Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis under various physiological and pathological conditions. We found that the VEGF isoforms VEGF120, VEGF164, and VEGF188 were expressed in the bovine mammary gland and bovine mammary epithelial cells (bMECs). Expression of VEGF in the mammary gland was significantly higher during the lactation period than during the dry period. Although dexamethasone or prolactin alone had little effect on the expression of VEGF, that in dexamethasone-treated cells was significantly induced after additional treatment with prolactin. Furthermore, the VEGF expression induced by the combination of dexamethasone and prolactin was reduced by PD98059 in a dose-dependent manner. This combination also stimulated the phosphorylation of p44/p42 MAP kinase in these cells. These results strongly suggest that the combination of dexamethasone and prolactin stimulates VEGF expression in bMECs via p44/p42 MAP kinase.

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References

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