Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Phylogeny of the family Ophioglossaceae with special emphasis on genus Mankyua

제주고사리삼을 중심으로한 고사리삼과 식물의 계통

  • Sun, Byung-Yun (Division of Biological Sciences, Chonbuk National University) ;
  • Baek, Tae Gyu (Division of Biological Sciences, Chonbuk National University) ;
  • Kim, Young-Dong (Division of Biological Sciences, Hallym University) ;
  • Kim, Chan Soo (Warm-temperate Forest Research Center, Korea Forest Research Institute)
  • 선병윤 (전북대학교 생물과학부) ;
  • 백태규 (전북대학교 생물과학부) ;
  • 김영동 (한림대학교 생명과학과) ;
  • 김찬수 (국립산림과학원 난대산림연구소)
  • Published : 2009.09.30
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Abstract

Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

엽록체 rbcL gene의 염기서열과 포자의 형태를 바탕으로 고사리삼과 식물의 계통과 제주고사리삼속의 계통학적 위치를 추정하였다. 엽록체 DNA rbcL 염기서열의 분석 결과 고사리삼과는 뚜렷하게 고사리삼계보 (Botrychioid lineage)와 나도고사리삼계보 (Ophioglossoid lineage)의 두 군으로 구분되었다. 고사리삼계보에 있어 제주고사리삼속(Mankyua)은 Helmintostachys속과 함께 분계의 기저에서 고사리삼속(Botrychium)의 자매분류군으로 분지하였으나, 이들 고사리삼속(Botrychium), 제주고사리삼속(Mankyua), 및 Helmintostachys속 사이의 계통적 유연관계는 결정되지 못했다. 고사리삼속의 경우 Sceptridium아속과 Botrychium아속이 뚜렷한 단계통을 형성하였으나, Botrypus아속에 속한 분류군들은 고사리삼속의 기저에서 다른 고사리삼속 분류군의 자매분류군으로 분지하면서 단계통이 아닌 것으로 밝혀졌다. 나도고사리삼계보를 형성하는 나도고사리삼속(Ophioglossum)의 경우, Ophioglossum아속은 단계통을 형성하였으며, Ophioglossum아속에 대해 Cheiroglossa아속 및 Ophioderma 아속이 차례로 자매분류군으로 분지하였다. 염기서열 계통수, 포자의 형태 및 외부형태의 고찰에 있어 제주고사리삼속은 Helminthostachys와 유사하지만, 계통수에서 patristic distance 뿐 아니라 포자소엽의 형태가 뚜렷이 구분되어 이들 2속은 독립된 속으로 판단된다.

Keywords

Acknowledgement

Supported by : 학술진흥재단

References

  1. Clausen, R. T. 1938. A monograph of the Ophioglossaceae. Mem. Torrey Bot. Club 19: 1-177
  2. Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15
  3. Eames, A. J. 1976. Morphology of Vascular Plants: Lower Groups (Psilophytales to Filicales). R. E. Krieger Pub. Co., New York
  4. Erdtman, G. 1954. An Introduction to Pollen Analysis. Chronica Botanica Co. P. 204
  5. Erdtman, G. 1966. Pollen Morphology and Plant Taxonomy. Angiosperms- An Introduction to Palynology. I - Hafner Publishing Co., N.Y., USA
  6. Farris, J., V. Albert., M. Kallersjo, D. Lipscomb and A. Kluge. 1996. Parsimony jackknifing outperforms neighbor-joining. Cladistics 12: 99-124 https://doi.org/10.1111/j.1096-0031.1996.tb00196.x
  7. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evol. 39: 783-791 https://doi.org/10.2307/2408678
  8. Gifford, E. M. and A. S. Foster. 1989. Morphology and Evolution of Vascular Plants, 3rd ed. W. H. Freeman and Company, New York
  9. Hasebe, M., M. Ito, R. Kofuju, K. Ueda and K. Iwatsuki. 1993. Phylogenetic relationships of ferns deduced from rbcL gene sequence. J. Mol. Evol. 37: 476-482 https://doi.org/10.1007/BF00160428
  10. Hasebe, M., P. G. Wolf, K. M. Pryer, K. Ueda, M. Ito, R. Sano, G. J. Gastony, J. Yokoyama, J. R. Manhart, N. Murakami, E. H. Crane, C. H. Haufler and W. D. Hauk. 1995. Fern phylogeny based on rbcL nucleotide sequences. Amer. Fern Jour. 85: 134-181 https://doi.org/10.2307/1547807
  11. Hauk, W. D., C. R. Parks and M. W. Chase. 2003. Phylogenetic studies of Ophioglossaceae: evidence from rbcL and trnL-F plastid DNA sequences and morphology. Mol. Phylogenet. Evol. 28: 131-151 https://doi.org/10.1016/S1055-7903(03)00032-0
  12. Kato, M. 1987. A phylogenetic classification of Ophioglossaceae. Gard. Bull. Sing. 40: 1-14
  13. Kato, M. 1995. Ophioglossaceae. In Flora of Japan, vol. 1. Pteridophytes and gymnospermae. Iwatsuki, K., T. Yamasaki, D. E. Boufford and H. Ohba (eds.), Kodansha, Tokyo. Pp. 22-28
  14. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparable studies of nucleotide sequences. J. Mol. Evol. 16: 111-120 https://doi.org/10.1007/BF01731581
  15. Lee, W. T. 1996. Lineamenta Florae Koreae. Academy press, Seoul, Korea. Pp. 12-16 (in Korean)
  16. Mabberley, D. J. 1990. The Plant-Book. Cambridge Univ. Press, Cambridge
  17. Nishida, M. 1952. A new system of Ophioglossales. J. Jap. Bot. 27: 271-278
  18. Pryer, M. K., H. Schneider, A. R. Smith, R. Cranfill, P. G. Wolf, J. S. Hunt and S. D. Sipes. 2001. Horsetails and ferns are monophyletic group and the closest living relatives to seed plants. Nature 409: 618-622 https://doi.org/10.1038/35054555
  19. Saitou, N. and M. Nei. 1987. The neighbour-joining method: A new method of reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425
  20. Smith, A. R., K. M. Pryer, E. Schuettpelz, P. Korall, H. Schneider and P. G. Wolf. 2006. A classification for extant ferns. Taxon 55: 705-731 https://doi.org/10.2307/25065646
  21. Sun, B. Y., M. H. Kim, C. H. Kim and C.-W. Park. 2001. Mankyua (Ophioglossaceae): a new fern genus from Cheju Island, Korea. Taxon 50: 1019-1024 https://doi.org/10.2307/1224718
  22. Swofford, D. L. 2002. PAUP$^{\ast}$. Phylogenetic analysis using parsimony ($^{\ast}$and Other Methods). Ver. 4.0b. Sinauer Associates, Sunderland, Massachusetts
  23. Tryon, A. F. and B. Lugardon. 1991. Spores of the Pteridophyta. Springer-Verlag, New York, USA. Pp. 28-37
  24. Wagner, W. H. 1990. Ophioglossaceae. In The Families and Genera of Vascular Plants: Ptreridophytes and Gymnosperms, vol. 1. Pteridophytes and Gymnosperms. Kramer, K. U. and P. S. Green (eds.), Springer-Verlag, New York. Pp. 193-197