• Korean Journal of Plant Taxonomy
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• v.36 no.1
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• pp.1-19
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• 2006

Description of spore morphology of Korean Polypodiaceae has on LM and SEM and key to the genera are provided. Korean Polypodiaceae monad, monolete with bilateral symmetry, bipolar and oblete spore except for Loxogramme grammitoides having trilete spore with radial symmetry. In spore wall structure, exopore showed variable patterns enough to distinguish genera. Perispore was found in all the species examined. The patternof perispore and exospore was most similar in all the species examined except Crypsinus, Pyrrosia hastata ans P. linearifolia. The perispores of Crypsinus, Pyrrosia hastata and P. linearifolia were echinate and gemmate, rugulate, and verrucate respectively. In term of exospore wall sculpturing, Polypodium and Loxogramme showed fossulate, Lepisorus, rugulate with irregular verrucate, Crypsinus ans Coloysis showed psilate or rarely verrucate in the latter, Neocheiropteris ensata showed dense small verrucate, Lemmaphyllum microphyllum showed complex reticulate and Pyrrosia showed verrucate or psilate.

• Journal of Plant Biology
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• v.37 no.1
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• pp.43-51
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• 1994

Spore morphology of 14 species of Ophioglossaceae is examined, and descriptions and key based on spore morphology are provided. In addition, spores of one species of Angiopteris and three species of Osmunda are examined for comparision with those of Ophioglossaceae. Spore shape at proximal face is sub triangular or triquete in Botrychium and circular in Ophiog~ lossum, Angiopteris and Osmunda. Based on the wall sculpturing patterns, three groups can be recognized within Botrychium, and the differences of wall sculpturing among these groups are smaller as compared to those among Botrychium, Ophioglossum, Osmunda and Angiopteris. This result supports the previous infrafamilial system of Clausen, which was mainly based on the characteristics of vernation, habit, fertile and sterile segments of the leaf, and gametophyte. Spores of nine species of Ophioglossum, which are circular in proximal face and fossulate or foveolate in wall sculpturing, are quite uniform as compared to those of Botrychium. Therefore they should be kept under a single genus rather than split into four genera suggested by Nishida (1952).(1952).

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.335.3-336
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• 2002

To find ${\alpha}$-Glucosidase Inhibitors produced by Actinomycetes, 20 soil samples were tested and 53 Actimycetes were isolated. One of 53 Actinomycetes (strain PM718) showed very potent inhibitory activity in vitro. The morphological and physiological characteristics of strain PM 718 were investigated. The spore morphology. spore chain morphology and spore surface were observed by scanning electron microscope. The inhibitory activity of strain PM718 in vivo has been studied in mice made hyperglycemia by Streptozotocin treatment. The strain PM718 showed signficant reduction of blood glucose level(more than 30%) in mice loaded with maltose.

• ALGAE
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• v.19 no.2
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• pp.91-105
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• 2004

Ten taxa of the genus Spirogyra (Zygnemataceae, Chlorophyta) in Korea were examined for morphology and taxonomic category with field as well as cultured materials: S. africana (Fritsch) Czurda, S. decimina (Muller) Kutzing, S. distenta Transeau, S. dubia Kutzing, S. ellipsospora Transeau, S. ellipsospora var. crassoidea Transeau, S. gracilis (Hassall) Kutzing, S. submajuscula Kutzing, S. peipingensis Jao and S. variformis Transeau. Features of vegetative cell and female gametangium, shape of septum, chloroplast number, sexuality, size and shape of zygospore, and ornamentation of spore wall were major characteristics used for determination of the species. All of them were described for the first time in Korea.

• The Korean Journal of Mycology
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• v.20 no.3
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• pp.222-228
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• 1992

Pleurotus florida was transformed by complementation of auxotrophic mutant using chimeric plasmid containing Flammulina velutipes leu 2 gene and pBR 322 replicon. Mycelial morphology of transformants was grown and compared on mushroom complete and minimal medium. Transformants were mated with monokaryon and their genetic recombination was investigated for the morphology of fruitbody and spore analysis. $Leu^+$ transformant showed same mating type of $A_1B_1$ as to the untransformed mutant. The transformant and the untransformed mutant were mated with monokaryon of which mating type is $A_2B_1$, respectively. Although fruitbody of the untransformed mutant was not produced, $leu^+$ transformant produced fruitbody. Spore analysis showed that leucine requiring spores from fruitbody of $leu^+$ transformant were diminished when compared with those of untransformed mutant.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1721-1726
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• 2007

Comparative morphology among species of the genus Calostoma, including C. cinnabarina, C. ravenelii, and C. japonicum, was investigated by scanning electron microscopy and atomic force microscopy. Spore morphology of C. cinnabarina and C. ravenelii showed no dramatic differences by light microcopy and scanning electron microscopy. To differentiate these species, atomic force microscopy was employed. Quantitative analysis of the surface roughness of basidiospores revealed subtle differences in height fluctuation at the nanometer scale between the species of Calostoma. Basidiospores of C. cinnabarina had a relatively rougher surface than those of C. ravenelii at $2.0{\times}2.0\;{\mu}m^2$ scan areas.

• Mycobiology
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• v.46 no.3
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• pp.278-282
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• 2018

Chrysanthemum coronarium is an economically important plant in Asia, and used medicinally, ornamentally and as a vegetable. In April 2017, leaf spot disease on C. coronarium was observed in Shiyan, Hubei, China. A single-spore isolate was obtained and identified based on morphology and sequence analysis using four regions (rDNA ITS, GAPDH, $EF-1{\alpha}$, and RPB2). The results indicated that the fungus is Alternaria argyranthemi. The pathogenicity tests revealed that the species could cause severe leaf spot and blight disease on the host. This is the first report of leaf spot disease on C. coronarium caused by A. argyranthemi in the world, which is also a new record of Alternaria species in China.

• The Korea Journal of Herbology
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• v.35 no.1
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• pp.9-18
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• 2020

Objectives : We tried to establish standards for genuine by discriminating 3 kinds of pollen and spore herbs that are highly to be mixed with each other. Using an scanning electron microscope, we prepare a standard for discriminating external morphological characters of minute herbs, thereby contributing to the stable supply of genuine herbal medicines. Methods : Standard samples were confirmed by literature review on external morphological characteristics of original plants and herbal medicines, and collection and identification of original plants. The herbal medicines on the market were purchased and classified with using naked eye observation and magnifier. Finally, micromorphological identifications were conducted using an scanning electron microscope. Results : 1. Pini Pollen was clearly distinguished by its relatively medium size and a pollen grain with two swollen reticulate sacci at both ends. The verrucate ornamentation on the exine surface of the corpus and a sunken leptoma germ pore may be used as a discrimination criteria. 2. Typhae Pollen was distinguished by its relatively small size and a saccus on the end of a pollen grain. Reticulate ornamentation of exine surface of the corpus, and a slightly clear ulcerate germ pore can be used as a discrimination criteria. 3. Lygodii Spora was distinguished by its relatively large triangular-ovate shape and trichotomous fissure. Verrucate-tuberculate ornamentation of exine surface and trilete aperture could be used as a discrimination criteria. Conclusion : These results indicate that the use of electron microscopy is very effective for discriminating the external morphology of minute herbal medicines.

• Applied Microscopy
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• v.13 no.1
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• pp.63-70
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• 1983

The cell division of Bacillus thuringiensis are studed by a electron microscope. It was observed that when the cell division was occurred, the bacterial transverse septeum was centripetally formed, and the bacterial spore was divided into two daughter cells. The fore spore septum was initiated by invagination from either sides of the cell membranes, and was easily distinguished it from the transverse septum of the vegetative cell division. The large vesicular mesosome was. observed at one end of the cell membrane. The nucleoids were of variously irregular shapes and had no a nuclear membrane. The morphology of the bacteria was visualizd by a scanning electron microscope. The surface of the cell was generally rough and had a single polar flagellum, which was appeared to be $0.2{\mu}$ in width and $13{\mu}$ in length.

• The Korean Journal of Quaternary Research
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• v.17 no.2
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• pp.39-41
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• 2003

Pollen and spores as well as their morphological characteristics are biological stability materials in which contains historical information about vegetation and climate changes. As noted researcher Neishtadt in 1971, the lake and swamp deposits were developed only in Holocene period. The geological history of Darkhad depression which is situated in the Northern end of Mongolia from ancient time become under scientific interest of many researchers. Our investigation was focused on pollen analysis of lake sediment of Darkhad depression which caused bottom of paleolake. In Mongolia the palynology science is beginning to develop since 1990 and in 2000 the laboratory of palynology was established at the department of Biology, State Pedagogical University of Mongolia. Researchers from this laboratory working on pollen morphology of present flora in order to classify plants taxonomy. Another part of our investigation is mellitopalynology, which is studying of pollen grains in a honey. Although, this research has been extending by pollen analysis of sediment and lake deposits in past few years on the basis of high sensitive microscope and modem technology.