Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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COMPARISON OF ANTIBACTERIAL EFFECT OF Listerine(R) WITH VARIOUS ROOT CANAL IRRIGANTS

수종의 근관세척액과 Listerine(R)의 항균성 비교 연구

  • Kim, Young-Hun (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University, Consevative dentisty) ;
  • Kang, Min-Kyung (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University, Consevative dentisty) ;
  • Choi, Eun-Kyoung (Dept. of Oral microbiology, Chonnam National University, Consevative dentisty) ;
  • Yang, So-Young (Dept. of Oral Anatomy, School of Dentistry, Chonnam National University, Consevative dentisty) ;
  • Yang, In-Seok (Dept. of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Kang, In-Chol (Dept. of Oral microbiology, Chonnam National University, Consevative dentisty) ;
  • Hwang, Yun-Chan (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University, Consevative dentisty) ;
  • Hwang, In-Nam (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University, Consevative dentisty) ;
  • Oh, Won-Mann (Dept. of Conservative Dentistry, School of Dentistry, Chonnam National University, Consevative dentisty)
  • 김영훈 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 강민경 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 최은경 (전남대학교 치의학 전문대학원 구강미생물학교실) ;
  • 양소영 (전남대학교 치의학 전문대학원 구강해부학교실) ;
  • 양인석 (서울대학교 치의학전문대학원 보존학교실) ;
  • 강인철 (전남대학교 치의학 전문대학원 구강미생물학교실) ;
  • 황윤찬 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 황인남 (전남대학교 치의학 전문대학원 보존학교실) ;
  • 오원만 (전남대학교 치의학 전문대학원 보존학교실)
  • Published : 2009.11.30
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Abstract

The purpose of this study is to compare the antibacterial effect of $Listerine^{(R)}$ on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using $Listerine^{(R)}$ as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and $Listerine^{(R)}$ were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of $Listerine^{(R)}$ and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p < 0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, $Listerine^{(R)}$ showed significantly low antibacterial effect compared with the other root canal irrigants (p < 0.05). In conclusion, the results reflect remarkably low antibacterial effect of $Listerine^{(R)}$ as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

본 연구는 여러 가지 근관세척액(NaOCl, CHX, EDTA)과 구강세정제로 사용되는 $Listerine^{(R)}$을 근관감염균주인 Porphyromonas gingivalis와 Enterococcus faecalis를 상대로 항균효과를 비교하고 $Listerine^{(R)}$이 근관세척액으로 사용가능한지를 확인하고자 시행하였다. 본 연구에서는 Porphyromonas gingivalis ATCC 3327과 Enterococcus faecalis ATCC 29212의 표준균주를 사용하였다. 실험을 위한 근관세척제로 0.1%, 0.2%, 1%, 2% Chlorhexidine(CHX)과 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.5M EDTA(18.6% EDTA). 그리고 $Listerine^{(R)}$원액을 이용하였다. 항균효과의 비교는 액체배지상에서 균주의 혼탁도와 한천 확산법을 이용한 억제대 비교로 하였다. 모든 실험군은 대조군과 비교시 근관내 균주에 항균성을 나타냈다(p < 0.001). 모든 농도의 NaOCl, CHX, 그리고 EDTA는 실험균주에서 높은 항균성을 보였다. 모든 실험에서 $Listerine^{(R)}$은 다른 근관세척제에 비해 낮은 항균성을 보였다. 결론적으로, $Listerine^{(R)}$이 E. faecalis와 P. gingivalis에 대해 항균성을 보이나, 일반적으로 사용되는 근관세척 액에 비해서는 현저히 낮은 항균성을 나타냄으로 근관세척 액으로 사용은 적합하지 않음을 나타냈다.

Keywords

References

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