Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

EFFECT OF SOFT CHELATING IRRIGATION ON THE SEALING ABILITY OF GP/AH PLUS ROOT FILLINGS

Soft chelating irrigation이 GP/AH Plus로 충전된 근관의 sealing ability에 미치는 영향에 대한 평가

  • Yu, Yi-Suk (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University) ;
  • Kim, Tae-Gun (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University) ;
  • Lee, Kwang-Won (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University) ;
  • Yu, Mi-Kyung (Department of Conservative Dentistry, School of Dentistry, Chonbuk National University)
  • 유이숙 (전북대학교 치과대학 치과보존학 교실) ;
  • 김태균 (전북대학교 치과대학 치과보존학 교실) ;
  • 이광원 (전북대학교 치과대학 치과보존학 교실) ;
  • 유미경 (전북대학교 치과대학 치과보존학 교실)
  • Published : 2009.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate the effect of soft chelating irrigant on the sealing ability of root fillings by using a glucose leakage test. A total of 45 single-rooted teeth were selected for the study. The teeth were decoronated leaving a total length of 13mm. The root canals prepared using K3 NiTi rotary instruments to an apical dimension of size 45(0.06 taper). The specimens were then randomly divided into 3 experimental groups of 13 roots each and 2 control groups of 3 roots each. Specimen in each group were prepared with different irrigation protocols : group 1, 2.5% NaOCl; group 2, 2.5% NaOCl and 17% EDTA: group 3, 2.5% NaOCl and 15% HEBP. The root canals were filled with gutta-percha and AH Plus sealer using lateral condensation. After 7 days in $37^{\circ}C$, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometry at 1, 4, 7, 14, 21 and 28 days. There was a tendency of increase in leakage in all experimental groups during experimental period. HEBP-treated dentin showed no significant difference with EDTA-treated dentin during experimental period. From the 21th day onward, HEBP-treated dentin showed significantly lower leakage than smear-covered dentin. HEBP-treated dentin displayed a similar sealing pattern to EDTA-treated dentin and a better sealing ability than smear-covered dentin. Consequently, a soft chelator(HEBP) could be considered as the possible alternative to EDTA.

본 연구의 목적은 glucose leakage test를 이용하여 soft chelating irrigation이 근관 충전의 sealing ability에 미치는 영향을 평가하는 것이다. 발치된 45개의 단근치를 수집하여 치관부를 잘라내 치근이 총 13mm가 되게 하였다. 근관은 K3 NiTi 구동 기구를 사용하여 성형하고 #45/.06 taper까지 확대하였다. 3개의 실험군(n=13)과 2개의 대조군(n=3)으로 나누었다. 실험군은 다음의 세척 방법으로 처리하였다. 1군, 2.5% NaOCl로 세척; 2군, 2.5% NaOCl로 세척 후 17% EDTA로 최종 세척; 3군, 2.5% NaOCl과 15% HEBP 혼합 용액으로 세척, 근관은 gutta-percha와 AH Plus를 사용하여 측방가압으로 충전하였다. $37^{\circ}C$, 습도 100%에서 7일 동안 보관하고 glucose leakage model을 이용하여 치관부로부터 치근부 방향의 미세누출을 정량화하였다. 1, 4, 7, 14, 21, 28일 째 누출된 glucose의 농도를 spectrophotometry로 측정하였다. 분석 결과 모든 실험군에서 실험 기간 동안 누출이 증가하는 경향이 있었다. HEBP처리군은 실험 기간 동안 EDTA처리군과 유의한 차이를 보이지 않았다. HEBP처리군은 21일 이후부터 도말층으로 덮인 NaOCl처리군에 비해 유의하게 낮은 누출을 보였다. HEBP로 처리된 상아질은 EDTA로 처리된 상아질과 비슷한 양상의 폐쇄를 보였으나 도말층이 남아있는 상아질보다는 우수한 sealing ability를 나타냈다. 그러므로 약한 킬레이트제인 HEBP는 EDTA의 대안이 될 수 있을 것이다.

Keywords

References

  1. Czonstkowsky M, Wilson EG, Holstein FA. The smear layer in endodontics. Dent Clin North Am 34:13-25, 1990
  2. Pashley DH, Michelich V, Kehl T.J. Dentin permeability: effects of smear layer removal. Dent Res 59:1398-1403, 1980 https://doi.org/10.1177/00220345800590080701
  3. Pashley DH. Smear layer: Physiological considerations. Operative Dentistry Supplement 3, 13-29, 1984
  4. Foster KH, Kulild JC, Weller RN. Effect of smear layer removal on the diffusion of calcium hydroxide through radicular dentin. J Endod 19:136-140, 1993 https://doi.org/10.1016/S0099-2399(06)80508-X
  5. White RR, Goldman M, Lin PS. The influence of the smeared layer upon dentinal tubule penetration by plastic filling materials. J Endod 10:558-562, 1984 https://doi.org/10.1016/S0099-2399(84)80100-4
  6. Oksan T, Aktener BO, Sen BH, Tezel H. The penetration of root canal sealers into dentinal tubules. Int Endod J 26:301-305, 1993 https://doi.org/10.1111/j.1365-2591.1993.tb00575.x
  7. Economides N, Liolios E, Kolokuris I, Beltes P. Longterm evaluation of the influence of smear layer removal on the sealing ability of different sealers. J Endod 25:123-5, 1999 https://doi.org/10.1016/S0099-2399(99)80010-7
  8. 이정민, 박상혁, 최기운. Smear layer 처리에 따른 미세누출에 대한 연구. 대한치과보존학회지 31:378-389, 2006 https://doi.org/10.5395/JKACD.2006.31.5.378
  9. Johnson WT, Gutmann JL. Obturation of the cleaned and shaped root canal system. In: Cohen S, Hargreaves KM, eds. Pathways of the Pulp, 9th edn. St Louis, MO: Mosby Elsevier, pp.358-99, 2005
  10. Shahravan A, Haghdoost AA, Adl A, Rahimi H, Shadifar F. Effect of smear layer on sealing ability of canal obturation: a systematic review and meta-analysis. J Endod 33:96-105, 2007 https://doi.org/10.1016/j.joen.2006.10.007
  11. Lester KS, Boyde A. Scanning electron microscopy of instrumented, irrigated and filled root canals. Br Dent J 143:359-367, 1977 https://doi.org/10.1038/sj.bdj.4804007
  12. Hulsmann N, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 36:810-830, 2003 https://doi.org/10.1111/j.1365-2591.2003.00754.x
  13. Zehnder M, Schmidlin P, Sener B, Waltimo T. Chelation in root canal therapy reconsidered. J Endod 31:817-820, 2005 https://doi.org/10.1097/01.don.0000158233.59316.fe
  14. Grawehr M, Sener B, Waltimo T, Zehnder M. Interaction of ethylenediamine tetracetic acid with sodium hypochlorite in aqueous solutions. Int Endod J 36:411-415, 2003 https://doi.org/10.1046/j.1365-2591.2003.00670.x
  15. Baumgartner JC, Marder CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod 13:147-157, 1987 https://doi.org/10.1016/S0099-2399(87)80132-2
  16. De-Deus G, Zehnder M, Reis C, Fidel S, Fidel RA, Galan J Jr, Paciornik S. Longitudinal co-site optical microscopy study on the chelating ability of etidronate and EDTA using a comparative single-tooth model. J Endod 34:71-75, 2008 https://doi.org/10.1016/j.joen.2007.09.020
  17. Ari H, Erdemir A, Belli S. Evaluation of the effect of endodontic irrigation solution on the microhardness and the roughness of root canal dentin. J Endod 30:792-795, 2004 https://doi.org/10.1097/01.DON.0000128747.89857.59
  18. De-Deus G, Namen F, Galan J Jr, Zehnder M. Soft chelating irrigation protocol optimizes bonding quality of Resilon/Epiphany root fillings. J Endod 34:703-705, 2008 https://doi.org/10.1016/j.joen.2008.02.024
  19. Russell RG, Gogers MJ. Bisphosphonates: from the laboratory to the clinic and back again. Bone 25:97-106, 1999 https://doi.org/10.1016/S8756-3282(99)00116-7
  20. Xu Q, Fan MW, Fan B, Cheung GS, Hu HL. A new quantitative method using glucose for analysis of endodontic leakage. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:107-111, 2005 https://doi.org/10.1016/j.tripleo.2004.06.006
  21. Shemesh H, Wu MK, Wesselink PR. Leakage along apical root fillings with and without smear layer usting two different leakage models: a two-month longitudinal ex vivo study. Int Endod J 39:968-976, 2006 https://doi.org/10.1111/j.1365-2591.2006.01181.x
  22. Wu M. K., Wesselink P. R. Endodontic leakage studies reconsidered. Part I. Methodology, Application and relevance. Int endod J 26:37-43, 1993 https://doi.org/10.1111/j.1365-2591.1993.tb00540.x
  23. Al-Ghamdi A, Wennberg A. Testing of sealing ability of endodontic filling materials. Endod Dent Traumatol 10:249-255, 1994 https://doi.org/10.1111/j.1600-9657.1994.tb00079.x
  24. Pommel L, Camps J. Effects of pressure and measurement time on the fluid filtration method in endodontics. J Endod 27:256-258, 2001 https://doi.org/10.1097/00004770-200104000-00003
  25. Rao P., Pattabiraman, T.N. Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentoses. Anal. Biochem. 189:18-22, 1989 https://doi.org/10.1016/0003-2697(89)90387-4
  26. Eldeniz AU, Erdemir A, Belli S. Shear bond strengths of three resin based sealers to dentin with and without the smear layer. J Endod 31:293-6, 2005 https://doi.org/10.1097/01.don.0000140577.99708.c8
  27. Behrend GD, Cutler CW, Gutmann JL. An in-vitro study of smear layer removal and microbial leakage along root-canal fiilings. Int Endod J 29:99-107, 1996 https://doi.org/10.1111/j.1365-2591.1996.tb01169.x
  28. De-Deus G, Soares J, Leal F, Luna AS, Fidel S, Fidel RA. Similar glucose leakage pattern on smear-covered, EDTA-treated and BioPure MTAD-treated dentin. J Endod 34:459-462, 2008 https://doi.org/10.1016/j.joen.2008.01.005
  29. 박동성. MTAD의 치근단 누출에 미치는 영향에 대한 전기화학적 연구. 대한치과보존학회지 31:119-124, 2006 https://doi.org/10.5395/JKACD.2006.31.2.119
  30. Shemesh H, Wu M-K, Wesselink PR. Leakage along apical root fillings with and without smear layer using two different leakage models: a two-month longitudinal ex vivo study. Int Endod J 39:968-976, 2006 https://doi.org/10.1111/j.1365-2591.2006.01181.x
  31. Calt S, Serper A. Smear layer removal by EGTA. J Endod 26:459-461, 2000 https://doi.org/10.1097/00004770-200008000-00007
  32. Eldeniz AU, Erdemir A, Belli S. Effect of EDTA and citric acid solutions on the microhardness and the roughness of human root canal dentin. J Endod 31: 107-10, 2005 https://doi.org/10.1097/01.don.0000136212.53475.ad
  33. Schwartz R. Adhesive dentistry and endodontics. Part 2: Bonding in the root canal system-The promise and the problems: A review. J Endod 32:1125-1134, 2006 https://doi.org/10.1016/j.joen.2006.08.003
  34. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by hostderived enzymes during aging. J Dent Res 83:216-21, 2004 https://doi.org/10.1177/154405910408300306
  35. Eldeniz AU, Erdemir A, Belli S. Shear bond strength of three resin based sealers to dentin with and without the smear layer. J Endod 31:293-296, 2005 https://doi.org/10.1097/01.don.0000140577.99708.c8
  36. K. Mamootil, H. H. Messer. Penetration of dentinal tubules by endodontic sealer cements in extracted teeth and in vivo. Int endod J 40: 873-881, 2007 https://doi.org/10.1111/j.1365-2591.2007.01307.x
  37. Tay FR, Hosoya Y, Loushine RJ, Pashley DH, Weller RN. Ultrastructure of intraradicular dentin after irrigation with BioPure MTAD. II. The consequence of obturation with an epoxy resin-based sealer. J Endod 32:473-477, 2006 https://doi.org/10.1016/j.joen.2005.10.054

Cited by

  1. Effect of moisture on sealing ability of root canal filling with different types of sealer through the glucose penetration model vol.35, pp.5, 2010, https://doi.org/10.5395/JKACD.2010.35.5.335