Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Triterpenoids from the Fruits of Cornus kousa Burg. as Human Acyl-CoA: Cholesterol Acyltransferase Inhibitors

  • Lee, Dae-Young (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Jung, La-Koon (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Lyu, Ha-Na (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Jeong, Tae-Sook (National Research Laboratory of Lipid Metabolism and Atherosclerosis, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Youn-Hyung (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
  • Published : 2009.02.28
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Abstract

The fruits of Cornus kousa Burg. were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was partitioned with ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$. From the EtOAc traction, 5 triterpenoids were isolated through repeated silica gel ($SiO_2$), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatography (c.c.). These compounds were determined to be ursolic acid (1), corosolic acid (2), taraxasterol (3), betulinic acid (4), and betulinic aldehyde (5) on the basis of their spectroscopic data including electronic ionization mass spectrometry, ultraviolet spectroscopy, infrared spectroscopy, and nuclear magnetic resonance. This is the first reported isolation of these compounds from this plant. Also, compounds 1, 3, 4, and 5 show a relatively high inhibitory activity against human acyl-CoA: cholesterol acyltransferase-1 (hACAT-1) with inhibition values of $52.8{\pm}0.7$, $91.1{\pm}0.4$, $93.0{\pm}0.7$, and $96.2{\pm}0.2%$ at a concentration of $100{\mu}M$, respectively.

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References

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