Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Effect of Prunella vulgaris Labiatae Extract on Innate Immune Cells and Anti-metastatic Effect in Mice

  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Ability of water extract from Prunella vulgaris Labiatae to stimulate immune system and inhibit tumor metastasis in mice was assessed. In experimental lung metastasis, prophylactic intravenous (i.v.) administration of water extract from P. vulgaris significantly inhibited lung metastasis in a dose-dependant manner. Peritoneal macrophages stimulated with P. vulgaris produced various cytokines such as tumor necrosis factor (TNF)-$\alpha$ and interlukin (IL)-12 as well as induced tumoricidal activity. In an assay for natural killer (NK) cell activity, i.v. administration of P. vulgaris significantly augmented NK cytotoxicity. The depletion of NK cells by injection of rabbit anti-asialo GM1 serum abolished the inhibitory effect of P. vulgaris on lung metastasis of colon26-M3.1 cells. These data demonstrate that P. vulgaris activate innate immune system to inhibit the growth of foreign materials including tumor cells in mice.

Keywords

References

  1. Villinger F. Cytokines as clinical adjuvants: How far are we? Expert Rev. Vaccines 2: 317-326 (2003) https://doi.org/10.1586/14760584.2.2.317
  2. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biot. 60:258-274 (2002) https://doi.org/10.1007/s00253-002-1076-7
  3. Chino A, Sakurai H, Choo MK, Koizumi K, Shimada Y, Terasawa K, Saiki I. Juzentaihoto, a Kampo medicine, enhances IL-12 production .by modulating toll-like receptor 4 signaling pathways in munne pentoneal exudate macrophages. Int. Immunopharmaco. 5:871-882 (2005) https://doi.org/10.1016/j.intimp.2005.01.004
  4. Colombo MP, Trinchieri G. Interleukin-12 in anti-tumor immunity and immunotherapy. Cytokine Growth F. R. 13: 155-168 (2002) https://doi.org/10.1016/S1359-6101(01)00032-6
  5. Yoon n, Yoo YC, Lee SW, Shin KS, Choi WH, Hwang SH, Ha ES, Jo SK, Kim SH, Park WM. Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J. Ethnopharmacol. 93: 247-253 (2004) https://doi.org/10.1016/j.jep.2004.03.052
  6. Saiki I, Saito S, Fujita C, Ishida H, Iida J, Murata J, Hasegawa A, Azuma I. Induction of tumoricidal macrophages and production of cytokines by synthetic muramyl dipeptide analogues. Vaccine 6:238-244 (1988) https://doi.org/10.1016/0264-410X(88)90218-6
  7. Fisher M, Yang LX. Anticancer effects and mechanisms of polysaccharide-K (PSK): Implications of cancer immuno-therapy. Anticancer Res. 22: 1737-1754 (2002)
  8. Efferth T, Kaha S, Paulus K, Adams M, Rauh R, Boechzelt H, Hao X, Kaina B, Bauer R. Phytochemistry and pharmacogenomics of natural products derived from traditional Chinese medicine and Chinese materia medica with activity against tumor cells. Mol. Cancer Ther. 7: 152-162 (2008) https://doi.org/10.1158/1535-7163.MCT-07-0073
  9. Rubin H. The significance of biological heterogeneity. Cancer Metast. Rev. 1: 1-20 (1990) https://doi.org/10.1007/BF00047585
  10. Schantz SP, Brown BW, Lira E, Taylor SL, Beddingfield N. Evidence for the role of natural immunity in the control of metastatic spread of head and neck cancer. Cancer Immunol. Immun. 25: 141-148 (1987) https://doi.org/10.1007/BF00199955
  11. An HJ, Um JY, Na HJ, Jeong S, Kim HM, Hong SH. Activation of inducible nitric oxide synthase by Kagamjuaguiem in peritoneal macrophages in mice. Indian J. Med. Res. 125: 740-746 (2007)
  12. Azuma I, Seya T. Development of immunoadjuvants for immunotherapy of cancer. Int. Immunopharmaco. 1: 1249-1259 (2001) https://doi.org/10.1016/S1567-5769(01)00055-8
  13. Sung JY, Yoon TJ, Yu KW, Lee KH, Lee H. Enhancement of immunological activities in mice by oral administration of pectic polysaccharides from Eleutherococus senticosus. Food Sci. Biotechnol. 15: 117-121 (2006)
  14. Lee SJ, Saiki I, Hayakawa Y, Nunome S, Yamada H, Kim SH. Antimetastatic and immunomodulating properties of a new herbal prescription, Bojung-bangam-tang. Int. Immunopharmaco. 3: 147-157 (2003) https://doi.org/10.1016/S1567-5769(02)00091-7
  15. Hunter CA, Chizzonite R, Remington JS. IL-1$\beta$ is required for IL-12 to induce production of IFN-$\gamma$ by NK cells. A role for IL-1$\beta$ in the T cell-independent mechanism of resistance against intracellular pathogens. J. Immunol. 155: 4347-4354 (1995)
  16. Kayagaki N, Yamaguchi N, Nakayama M, Takeda K, Akiba H, Tsutsui H, Okamura H, Nakanishi K, Okumura K, Yagita H. Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells. J. Immunol. 163: 1906-1913 (1999)
  17. Yoon TJ, Yoo YC, Kang TB, Baek YJ, Huh CS, Song SK, Lee KH, Azuma I, Kim JB. Prophylactic effect of Korean mistletoe (Viscum album coloratum) extract on tumor metastasis is mediated by enhancement of NK cell activity. Int. J. Immunopharmacol. 20: 163-172 (1998) https://doi.org/10.1016/S0192-0561(98)00024-1
  18. Fang X, Chang RC, Yuen WH, Zee SY. Immune modulatory effects of Prunella vulgaris L. Int. J. Mol. Med. 15: 491-496 (2005)
  19. Kim SY, Kim SH, Shin HY, Lim JP, Chae BS, Park JS, Hong SG, Kim MS, Jo DG, Park WH, Shin TY. Effects of Prunella vulgaris on mast cell-mediated allergic reaction and inflammatory cytokine production. Exp. BioI. Med. 232: 921-926 (2007)
  20. Fang X, Yu MM, Yuen WH, Zee SY, Chang RC. Immune modulatory effects of Prunella vulgaris L. on monocytes/macrophages. Int. J. Mol. Med. 16: 1109-1116 (2005)
  21. Pope BL, Sigindere J, Chourmouzis E, MacIntyre P, Goodman MG. 7-Allyl-8-oxoguanosine (loxoribine) inhibits the metastasis of B16 melanoma cells and has adjuvant activity in mice immunized with a B16 tumor vaccine. Cancer Immunol. Immun. 38: 83-91 (1994) https://doi.org/10.1007/BF01526202
  22. Tanigawa K, Craig RA, Stoolman LM, Chang AE. Effects of tumor necrosis factor-alpha on the in vitro maturaton of tumor-reactive effector T cells. J. Immunother. 23: 528-535 (2000) https://doi.org/10.1097/00002371-200009000-00003
  23. Lasek W, Feleszko W, Golab J, Stoklosa T, Marczak M, Dabrowska A, Malejczyk M, Jakobisiak M. Antitumor effects of the combination immunotherapy with interleukin-12 and tumor necrosis factor alpha in mice. Cancer Immunol. Immun. 45: 1000-1008 (1997)
  24. Baxevanis CN, Voutsas IF, Tsitsilonis OE, Tsiatas ML, Gritzapis AD, Papamichail M. Compromised anti-tumor responses in tumor necrosis factor-alpha knockout mice. Eur. J. Immunol. 30: 1957-1966 (2000) https://doi.org/10.1002/1521-4141(200007)30:7<1957::AID-IMMU1957>3.0.CO;2-7
  25. Markovic SN, Murasko DM. Role of natural killer and T-cells in interferon induced inhibition of spontaneous metastases of the B16F10L murine melanoma. Cancer Res. 51: 1124-1128 (1991)
  26. Kim R, Emi M, Tanabe K. Cancer immunoediting from immune surveillance to immune escape. Immunology 121: 1-14 (2007) https://doi.org/10.1111/j.1365-2567.2007.02587.x