Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Rapid Identification of Cow and Goat Milk in Milk Products Using a Duplex PCR Technique

Duplex PCR을 이용한 유제품 안에 있는 산양유와 우유의 신속한 동정에 대한 연구

  • Lee, Seung-Bae (Division of Animal Resources and Life Science, Sangji University) ;
  • Choi, Suk-Ho (Division of Animal Resources and Life Science, Sangji University)
  • 이승배 (상지대학교 동물생명자원학부) ;
  • 최석호 (상지대학교 동물생명자원학부)
  • Published : 2009.10.31
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Abstract

A duplex PCR technique was applied for specific identification of cow and goat milk in milk products by using primers targeting the mitochondrial 12S rRNA gene. Duplex PCR using primers specific for cow and goat generated specific fragments of 223bp and 326bp from cow and goat milk DNA, respectively. Duplex PCR was applied to 15 milk products purchased from the market to verify label statements. The labeling statements of four market milk products, three yoghurt products, and one whole milk powder product were confirmed in the duplex PCR. The labeling statements of five of seven infant milk powder products were also confirmed by duplex PCR but the other two products were shown to be contaminated with either cow or goat milk. The proposed duplex PCR provides a rapid and sensitive approach to detection of as little as 0.1% cow milk in goat milk and one-step detection of cow or goat milk in milk products.

유제품에 들어 있는 우유와 산양유를 동정하기 위해 미토콘드리아의 12S rRNA 유전자를 목표로 하는 primer을 이용하는 duplex PCR 분석을 적용하였다. 소와 산양의 특이성 primer을 이용한 duplex PCR 분석은 우유와 산양유 DNA에 대해 각각 233 bp와 326 bp의 특이성 단편을 나타냈다. Duplex PCR 분석이 라벨에 표시된 성분을 확인하기위하여 시중마트에서 구입한 15개 유제품에 적용하였다. Duplex PCR 분석 결과 4개 시유, 3개 요구르트, 1개 전지분유는 표시된 성분과 완전히 일치하였다. 그러나 7개의 조제분유 중 5개만 표시성분과 일치하고 2개 조제분유제품은 산양유와 우유가 각각 오염되어 있는 것으로 나타났다. 제안된 duplex PCR 분석은 산양유에 들어있는 우유를 0.1%까지 측정할 수 있는 민감하고 신속한 방법이다. Duplex PCR 분석은 유제품 속에 들어있는 우유와 산양유를 one-step 방법으로 동시에 탐지할 수 있다.

Keywords

References

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