Mycobiology

The Korean Society of Mycology (한국균학회)
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Fungal Strain Improvement for Cellulase Production Using Repeated and Sequential Mutagenesis

  • Vu, Van-Hanh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Pham, Tuan-Anh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
  • Published : 2009.12.31
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Abstract

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of $\gamma$-irradiation of $Co^{60}$, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and $\beta$-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.

Keywords

References

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