Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Polysaccharide Fraction from Euonymus alatus Sieb on T-, B-Lymphocytes and Macrophages in Mice

귀전우 다당류 분획이 생쥐의 T 및 B 임파구와 대식세포의 활성에 미치는 영향

  • Published : 2008.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of polysaccharide fraction from Euonymus alatus Sieb(EPF) on the immune response of T-, B-lymphocytes and macrophages were examined in vitro and in vivo system. EPF (500 mg/kg) were administered p.o. twice a day for 5 days to C57BL/6 mice, and then the cells were separated from mice. EPF decreased the viability of thymocytes, but increased the viability of splenocytes in vitro and in vivo system. Also, the administration of EPF enhanced the population of helper T cell and cytotoxic T cell in thymocytes and did not affect the population of splenocytes. Furthermore, EPF enhanced the phagocytic activity and the production of nitric oxide in peritoneal macro phages in vivo system. These results suggest that EPF regulates an immune response via the enhancement of mature T lymphocytes and B lymphocytes viability and phagocytic activity of macrophages.

Keywords

References

  1. 생약학교재편찬위원회. 생약학, 동명사, p 224, 2005
  2. Huang, K.C. The pharmacology of Chinese herbs. Boca Raton: CRC Press, pp 93-94, 1993
  3. Kitanaka, S., Takido, M., Mizoue, K. and Nakaike, S. Cytotoxic cardenolides from woods of Euonymus alata, Chemical and Pharmaceutical Bulletin 44: 615-617, 1996 https://doi.org/10.1248/cpb.44.615
  4. Jin, U.H., Lee, J.Y., Kang, S.K., Kim, J.K., Park, W.H., Kim, J.G., Moon, S.K., Kim, C.H. A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 77(22):2760-2769, 2005 https://doi.org/10.1016/j.lfs.2005.02.028
  5. Park, W.H., Kim, S.H., Kim, C.H. A new matrix metalloproteinase-9 inhibitor 3,4-dihydroxycinnamic acid (caffeic acid) from methanol extract of Euonymus alatus: isolation and structure determination. Toxicology, 207(3):383-390, 2005 https://doi.org/10.1016/j.tox.2004.10.008
  6. Fang, X.K., Gao, Y., Yang, H.Y., Lang, S.M., Wang, Q.J., Yu, B.Y., Zhu, D.N. Alleviating effects of active fraction of Euonymus alatus abundant in flavonoids on diabetic mice. Am. J. Chin. Med. 36(1):125-140, 2008 https://doi.org/10.1142/S0192415X08005643
  7. Park, S.H., Ko, S.K., Chung, S.H. Euonymus alatus prevents the hyperglycemia and hyperlipidemia induced by high-fat diet in ICR mice. J Ethnopharmacol. 102(3):326-335, 2005 https://doi.org/10.1016/j.jep.2005.06.041
  8. Eun, J.S., Park, S.H., Kweon, J., Kim, Y.A., Kang, S.Y., Oh, C.H. and Jeon, H. Cytotoxicity of petroleum ether extract of Euonymus alatus. Kor. J. Pharmacogn. 26(3):253-258, 1995
  9. Lee, J.H., Kim, H.K. and Ha, T.Y. Antitumor effect of winged Euonymus against chemically induced and malignant cell implanted-tumors in mice. Kor. J. Immunol. 15(2):243-247, 1993
  10. Chung, H.S., Jeong, H.J., Kim, J.S., Jeong, S.I., Kim, K.S., Kim, K.S., Kang, B.K., Ahn, J.W., Baek, S.H., Kim, H.M. Activation of inducible nitric oxide synthase by Euonymus alatus in mouse peritoneal macrophages. Clin. Chim. Acta. 318(1-2):113-120, 2002 https://doi.org/10.1016/S0009-8981(01)00808-7
  11. Wysocki, L.J. and Sato, V.L. Planning for lymphocytes: A method for cell selection. Proc. Nat1. Acad. Sci. USA, 75: 2844-2848, 1978
  12. Mizel, S.B., Openheim, J.J., Rosensteich, D.L. Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. J. Immunol. 120: 1497-1503, 1979
  13. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. methods, 65: 55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  14. Kotnic, V., Fleischmann, W.R.Jr. A simple and rapid method to determine hematopoietic growth factor activity. J. Immunol. methods, 129: 23-30, 1990 https://doi.org/10.1016/0022-1759(90)90416-S
  15. Suda, T., Nagata, S. Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179: 873-879, 1994 https://doi.org/10.1084/jem.179.3.873
  16. Boudard, F., Vallot, N., Cabaner, C., Bastide, M. Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. J. Immunol. Methods, 174: 259-268, 1994 https://doi.org/10.1016/0022-1759(94)90030-2
  17. Blair, A.L., Cree, I.A., Beck, J.S., Hating, M.J.G. Measurement of phagocyte chemiluminenscence in a microtiter plate format. J. Immunol. Methods, 112: 163-168, 1988 https://doi.org/10.1016/0022-1759(88)90352-3
  18. Rockett, K.A., Awburn, M.M., Cowden, W.B., Clark, I.A. Killing of Plasmodium faciparum in vitro by nitric oxide derivatives. Infec. Immunity, 59(9):3280-3283, 1991
  19. Channon, J.Y., Leslie, C.C., Johnston, Jr.R.B. Zymosan- stimulated production of phosphatidic acid by macrophages: relationship to release of superoxide anion and inhibition by agents that increase intracellular cyclic AMP. J. Leucocyte Biol. 41: 450-455, 1987 https://doi.org/10.1002/jlb.41.5.450
  20. Miceli, M.C., Parnes, J.R. The role of CD4 and CD8 in T cell activation and differentiation. Advances in Immunology, 53: 59-63, 1993 https://doi.org/10.1016/S0065-2776(08)60498-8
  21. Abbas, A.K., Lichtman, A.H., Pober, J.S. Cellular and molecular immunology. Saunders Company(2ed). pp 177-178, 1994
  22. Breiheim, G., Stendahl, O., Dahlgren, C. Intra- and extracellular events in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Infect. Immun. 45: 1-8, 1984
  23. Nathan, C.F., Hibbs, J.B. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr. Opin. Immunol. 3: 65-70, 1991 https://doi.org/10.1016/0952-7915(91)90079-G
  24. Kilbourn, R.G., Griffith, O.W. Overproduction of nitric oxide in cytokine-mediated and septic shock. J. Natl. Cancer Instit. 84(11):828-831, 1992