Browse > Article

Effects of Polysaccharide Fraction from Euonymus alatus Sieb on T-, B-Lymphocytes and Macrophages in Mice  

Kang, Jae-Woo (College of Pharmacy, Woosuk University)
Shin, Ji-Seop (College of Pharmacy, Woosuk University)
Bang, Jun-Sung (College of Pharmacy, Woosuk University)
Jang, Sung-Hoon (College of Pharmacy, Woosuk University)
Li, Ri-Hua (College of Pharmacy, Woosuk University)
Jeon, Hoon (College of Pharmacy, Woosuk University)
Kim, Dae-Geun (College of Pharmacy, Woosuk University)
Oh, Chan-Ho (College of Science Technology, Woosuk University)
Cho, Hyoung-Kwon (Hanpoong Pharmaceutical Company)
Eun, Jae-Soon (College of Pharmacy, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.5, 2008 , pp. 1270-1275 More about this Journal
Abstract
Effects of polysaccharide fraction from Euonymus alatus Sieb(EPF) on the immune response of T-, B-lymphocytes and macrophages were examined in vitro and in vivo system. EPF (500 mg/kg) were administered p.o. twice a day for 5 days to C57BL/6 mice, and then the cells were separated from mice. EPF decreased the viability of thymocytes, but increased the viability of splenocytes in vitro and in vivo system. Also, the administration of EPF enhanced the population of helper T cell and cytotoxic T cell in thymocytes and did not affect the population of splenocytes. Furthermore, EPF enhanced the phagocytic activity and the production of nitric oxide in peritoneal macro phages in vivo system. These results suggest that EPF regulates an immune response via the enhancement of mature T lymphocytes and B lymphocytes viability and phagocytic activity of macrophages.
Keywords
Euonymus alatus Sieb(EPF); thymocytes; splenocytes; macrophages;
Citations & Related Records
연도 인용수 순위
  • Reference
1 생약학교재편찬위원회. 생약학, 동명사, p 224, 2005
2 Eun, J.S., Park, S.H., Kweon, J., Kim, Y.A., Kang, S.Y., Oh, C.H. and Jeon, H. Cytotoxicity of petroleum ether extract of Euonymus alatus. Kor. J. Pharmacogn. 26(3):253-258, 1995
3 Chung, H.S., Jeong, H.J., Kim, J.S., Jeong, S.I., Kim, K.S., Kim, K.S., Kang, B.K., Ahn, J.W., Baek, S.H., Kim, H.M. Activation of inducible nitric oxide synthase by Euonymus alatus in mouse peritoneal macrophages. Clin. Chim. Acta. 318(1-2):113-120, 2002   DOI
4 Wysocki, L.J. and Sato, V.L. Planning for lymphocytes: A method for cell selection. Proc. Nat1. Acad. Sci. USA, 75: 2844-2848, 1978
5 Suda, T., Nagata, S. Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179: 873-879, 1994   DOI   ScienceOn
6 Blair, A.L., Cree, I.A., Beck, J.S., Hating, M.J.G. Measurement of phagocyte chemiluminenscence in a microtiter plate format. J. Immunol. Methods, 112: 163-168, 1988   DOI   ScienceOn
7 Channon, J.Y., Leslie, C.C., Johnston, Jr.R.B. Zymosan- stimulated production of phosphatidic acid by macrophages: relationship to release of superoxide anion and inhibition by agents that increase intracellular cyclic AMP. J. Leucocyte Biol. 41: 450-455, 1987   DOI
8 Miceli, M.C., Parnes, J.R. The role of CD4 and CD8 in T cell activation and differentiation. Advances in Immunology, 53: 59-63, 1993   DOI
9 Nathan, C.F., Hibbs, J.B. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr. Opin. Immunol. 3: 65-70, 1991   DOI   ScienceOn
10 Breiheim, G., Stendahl, O., Dahlgren, C. Intra- and extracellular events in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Infect. Immun. 45: 1-8, 1984
11 Fang, X.K., Gao, Y., Yang, H.Y., Lang, S.M., Wang, Q.J., Yu, B.Y., Zhu, D.N. Alleviating effects of active fraction of Euonymus alatus abundant in flavonoids on diabetic mice. Am. J. Chin. Med. 36(1):125-140, 2008   DOI   ScienceOn
12 Boudard, F., Vallot, N., Cabaner, C., Bastide, M. Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. J. Immunol. Methods, 174: 259-268, 1994   DOI
13 Lee, J.H., Kim, H.K. and Ha, T.Y. Antitumor effect of winged Euonymus against chemically induced and malignant cell implanted-tumors in mice. Kor. J. Immunol. 15(2):243-247, 1993
14 Kotnic, V., Fleischmann, W.R.Jr. A simple and rapid method to determine hematopoietic growth factor activity. J. Immunol. methods, 129: 23-30, 1990   DOI   ScienceOn
15 Kitanaka, S., Takido, M., Mizoue, K. and Nakaike, S. Cytotoxic cardenolides from woods of Euonymus alata, Chemical and Pharmaceutical Bulletin 44: 615-617, 1996   DOI   ScienceOn
16 Mizel, S.B., Openheim, J.J., Rosensteich, D.L. Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. J. Immunol. 120: 1497-1503, 1979
17 Park, W.H., Kim, S.H., Kim, C.H. A new matrix metalloproteinase-9 inhibitor 3,4-dihydroxycinnamic acid (caffeic acid) from methanol extract of Euonymus alatus: isolation and structure determination. Toxicology, 207(3):383-390, 2005   DOI   ScienceOn
18 Abbas, A.K., Lichtman, A.H., Pober, J.S. Cellular and molecular immunology. Saunders Company(2ed). pp 177-178, 1994
19 Huang, K.C. The pharmacology of Chinese herbs. Boca Raton: CRC Press, pp 93-94, 1993
20 Kilbourn, R.G., Griffith, O.W. Overproduction of nitric oxide in cytokine-mediated and septic shock. J. Natl. Cancer Instit. 84(11):828-831, 1992
21 Park, S.H., Ko, S.K., Chung, S.H. Euonymus alatus prevents the hyperglycemia and hyperlipidemia induced by high-fat diet in ICR mice. J Ethnopharmacol. 102(3):326-335, 2005   DOI   ScienceOn
22 Rockett, K.A., Awburn, M.M., Cowden, W.B., Clark, I.A. Killing of Plasmodium faciparum in vitro by nitric oxide derivatives. Infec. Immunity, 59(9):3280-3283, 1991
23 Jin, U.H., Lee, J.Y., Kang, S.K., Kim, J.K., Park, W.H., Kim, J.G., Moon, S.K., Kim, C.H. A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 77(22):2760-2769, 2005   DOI   ScienceOn
24 Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. methods, 65: 55-63, 1983   DOI